Fassina Lorenzo, Saino Enrica, Visai Livia, Silvani Giulia, Cusella De Angelis Maria Gabriella, Mazzini Giuliano, Benazzo Francesco, Magenes Giovanni
Dipartimento di Informatica e Sistemistica, University of Pavia, Pavia, Italy.
J Biomed Mater Res A. 2008 Dec 1;87(3):750-9. doi: 10.1002/jbm.a.31827.
The surface properties of a biomaterial are fundamental to determine the response of the host tissue. In the present study, we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). Reverse transcriptase-polymerase analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-beta1. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.
生物材料的表面特性对于决定宿主组织的反应至关重要。在本研究中，我们采用了一种特定的仿生策略，即电磁刺激的人成骨细胞SAOS-2在钛纤维网表面增殖并形成钙化的细胞外基质。与对照条件相比，电磁刺激（磁场强度2 mT；频率75 Hz）导致更高的细胞增殖，并增加了I型胶原蛋白、核心蛋白聚糖和骨桥蛋白的表面覆盖（分别为9.8倍、11.3倍和9.5倍）。逆转录酶-聚合酶分析显示，电磁刺激上调了上述基质蛋白和生长因子TGF-β1的特异性转录。I型胶原蛋白、核心蛋白聚糖和骨桥蛋白的免疫荧光显示它们在细胞丰富区域共定位。使用电磁生物反应器旨在使生物相容性金属支架在细胞定植和钙化细胞外基质覆盖方面获得表面改性。表面改性的生物材料在临床应用中可作为骨修复植入物使用。
