Icaro Cornaglia Antonia, Casasco M, Riva F, Farina A, Fassina L, Visai L, Casasco A
Department of Experimental Medicine, Histology and Embryology Unit, via Forlanini 10, University of Pavia, Pavia, Italy.
Eur J Histochem. 2006 Jul-Sep;50(3):199-204.
The histogenesis of bone tissue is strongly influenced by physical forces, including magnetic fields. Recent advances in tissue engineering has permitted the generation of three dimensional bone-like constructs. We have investigated the effects of electromagnetic stimulation on human osteoblast cells grown in a hydrophobic polyurethane scaffold. Bone-like constructs were stimulated by pulsed electromagnetic fields in a bioreactor. Proliferation, bone protein expression and calcified matrix production by osteoblasts were measured using histochemical methods. In stimulated cultures, the number of cells was significantly higher compared to static (control) cultures. In both stimulated and control cultures, cells were immunoreactive to osteoblast markers, including type-I collagen, osteocalcin and osteopontin, thus suggesting that the expression of bone-related markers was maintained throughout the in vitro experiments. Morphometric analysis of von Kossa-stained sections revealed that stimulation with electromagnetic field significantly increased matrix calcification. The data lend support to the view that the application of a magnetic field can be used to stimulate cell growth in bone-like constructs in vitro. This finding may be of interest for the production of biomaterials designed for clinical applications.
骨组织的组织发生受到包括磁场在内的物理力的强烈影响。组织工程学的最新进展使得三维骨样构建体的生成成为可能。我们研究了电磁刺激对在疏水性聚氨酯支架中生长的人成骨细胞的影响。骨样构建体在生物反应器中受到脉冲电磁场的刺激。使用组织化学方法测量成骨细胞的增殖、骨蛋白表达和钙化基质产生。在受刺激的培养物中，细胞数量比静态（对照）培养物显著更高。在受刺激和对照培养物中，细胞对成骨细胞标志物（包括I型胶原蛋白、骨钙素和骨桥蛋白）均具有免疫反应性，因此表明在整个体外实验过程中骨相关标志物的表达得以维持。对冯·科萨染色切片的形态计量分析表明，电磁场刺激显著增加了基质钙化。这些数据支持了以下观点，即磁场的应用可用于在体外刺激骨样构建体中的细胞生长。这一发现可能对设计用于临床应用的生物材料的生产具有重要意义。
