Bloise Nora, Patrucco Alessia, Bruni Giovanna, Montagna Giulia, Caringella Rosalinda, Fassina Lorenzo, Tonin Claudio, Visai Livia
Department of Molecular Medicine (DMM), Centre for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli, 3/B-27100 Pavia, Italy.
Department of Occupational Medicine, Toxicology and Environmental Risks, Istituti Clinici Scientifici (ICS) Maugeri, IRCCS, Via Boezio, 28-27100 Pavia, Italy.
Materials (Basel). 2020 Jul 8;13(14):3052. doi: 10.3390/ma13143052.
Pulsed electromagnetic field (PEMF) has drawn attention as a potential tool to improve the ability of bone biomaterials to integrate into the surrounding tissue. We investigated the effects of PEMF (frequency, 75 Hz; magnetic induction amplitude, 2 mT; pulse duration, 1.3 ms) on human osteoblast-like cells (SAOS-2) seeded onto wool keratin scaffolds in terms of proliferation, differentiation, and production of the calcified bone extracellular matrix. The wool keratin scaffold offered a 3D porous architecture for cell guesting and nutrient diffusion, suggesting its possible use as a filler to repair bone defects. Here, the combined approach of applying a daily PEMF exposure with additional osteogenic factors stimulated the cells to increase both the deposition of bone-related proteins and calcified matrix onto the wool keratin scaffolds. Also, the presence of SAOS-2 cells, or PEMF, or osteogenic factors did not influence the compression behavior or the resilience of keratin scaffolds in wet conditions. Besides, ageing tests revealed that wool keratin scaffolds were very stable and showed a lower degradation rate compared to commercial collagen sponges. It is for these reasons that this tissue engineering strategy, which improves the osteointegration properties of the wool keratin scaffold, may have a promising application for long term support of bone formation in vivo.
脉冲电磁场（PEMF）作为一种潜在工具，可提高骨生物材料与周围组织整合的能力，已引起关注。我们研究了PEMF（频率75Hz；磁感应强度2mT；脉冲持续时间1.3ms）对接种在羊毛角蛋白支架上的人成骨样细胞（SAOS-2）在增殖、分化和钙化骨细胞外基质产生方面的影响。羊毛角蛋白支架为细胞寄居和营养物质扩散提供了三维多孔结构，表明其可能用作修复骨缺损的填充物。在此，每日PEMF暴露与额外成骨因子相结合的方法刺激细胞增加骨相关蛋白的沉积以及在羊毛角蛋白支架上的钙化基质。此外，SAOS-2细胞、PEMF或成骨因子的存在并不影响湿态下角蛋白支架的压缩行为或弹性。此外，老化试验表明，与商用胶原海绵相比，羊毛角蛋白支架非常稳定，降解率较低。正是由于这些原因，这种改善羊毛角蛋白支架骨整合特性的组织工程策略可能在体内长期支持骨形成方面具有广阔的应用前景。
