Hollister Jason R, Vagnozzi Ariel, Knowles Nick J, Rieder Elizabeth
Foreign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, NY 11944, USA.
Virology. 2008 Apr 10;373(2):411-25. doi: 10.1016/j.virol.2007.12.019. Epub 2008 Jan 16.
Bovine rhinovirus 2 (BRV2), a causative agent of respiratory disease in cattle, is tentatively assigned to the genus Rhinovirus in the family Picornaviridae. A nearly full-length cDNA of the BRV2 genome was cloned and the nucleotide sequence determined. BRV2 possesses a putative leader proteinase, a small 2A protein and a poly(C) tract, which are characteristic of aphthoviruses. Alignment of BRV-2 and FMDV polyproteins showed that 41% of amino acids were identical within the P1 region. Furthermore, 2A, 2C, 3B(3), 3C and 3D proteins are as much as 67%, 52%, 52%, 50%, and 64% identical, respectively. BRV2 leader protein is rapidly released from the viral polyprotein and cleaves eIF4G at a rate similar to FMDV leader proteinase, suggesting a functional relationship between the leader protein in these viruses. The results suggest that BRV2 is closely related to FMDV and should therefore be considered as a new species within the genus Aphthovirus.
牛鼻病毒2型（BRV2）是牛呼吸道疾病的病原体，暂归属于小RNA病毒科鼻病毒属。克隆了BRV2基因组的近全长cDNA并测定了核苷酸序列。BRV2具有一个推定的前导蛋白酶、一个小的2A蛋白和一个聚（C）序列，这些是口蹄疫病毒的特征。BRV-2和口蹄疫病毒多聚蛋白的比对显示，P1区域内41%的氨基酸相同。此外，2A、2C、3B(3)、3C和3D蛋白分别有67%、52%、52%、50%和64%的同源性。BRV2前导蛋白能迅速从病毒多聚蛋白中释放出来，并以与口蹄疫病毒前导蛋白酶相似的速率切割真核翻译起始因子4G，这表明这些病毒中的前导蛋白之间存在功能关系。结果表明，BRV2与口蹄疫病毒密切相关，因此应被视为口蹄疫病毒属内的一个新种。
