McFadden David W, Riggs Dale R, Jackson Barbara J, Cunningham Cynthia
Department of Surgery, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, USA.
Oncol Rep. 2008 Feb;19(2):563-6.
Inositol hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate that is found in food sources high in fiber content. IP6 has been reported to have significant inhibitory effects against a variety of primary tumors. We hypothesized that IP6 would inhibit the cell growth rate of Barrett's adenocarcinoma in vitro. Two Barrett's-associated adenocarcinoma cell lines, SEG-1 and BIC-1, were treated with IP6 at 0.5, 1.0 and 5.0 mM concentrations. Cell viability was measured by MTT assay. Apoptosis and necrosis were evaluated by the Annexin V FITC assay. Reductions (P<0.001) in cellular proliferation were observed in both cell lines. IP6 decreased late apoptosis and necrosis in BIC cells, whereas in SEG-1 cells, early apoptosis, late apoptosis and necrosis were all increased by IP6. IP6 decreases cellular growth by pro-apoptotic mechanisms. Our findings suggest that IP6 has the potential to become an effective adjunct for Barrett's adenocarcinoma. Further studies are needed to evaluate safety and clinical utility of this agent in patients with Barrett's adenocarcinoma.
肌醇六磷酸（IP6）是一种天然存在的多磷酸化碳水化合物，存在于高纤维含量的食物来源中。据报道，IP6对多种原发性肿瘤具有显著的抑制作用。我们假设IP6在体外会抑制巴雷特腺癌的细胞生长速率。用浓度为0.5、1.0和5.0 mM的IP6处理两种与巴雷特相关的腺癌细胞系SEG-1和BIC-1。通过MTT法测定细胞活力。通过膜联蛋白V FITC法评估细胞凋亡和坏死情况。在两种细胞系中均观察到细胞增殖减少（P<0.001）。IP6减少了BIC细胞中的晚期凋亡和坏死，而在SEG-1细胞中，IP6使早期凋亡、晚期凋亡和坏死均增加。IP6通过促凋亡机制降低细胞生长。我们的研究结果表明，IP6有潜力成为巴雷特腺癌的一种有效辅助治疗手段。需要进一步研究来评估该药物在巴雷特腺癌患者中的安全性和临床效用。
