Pandiri A R, Gimeno I M, Reed W M, Lee L F, Silva R F, Fadly A M
USDA ARS Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.
Avian Pathol. 2008 Feb;37(1):7-13. doi: 10.1080/03079450701774843.
Immunohistochemistry and polymerase chain reaction (PCR) were used to test for the presence of avian leukosis virus (ALV) J viral antigen gp85 and proviral DNA, respectively, in various tissues (adrenal gland, bone marrow, gonad, heart, kidney, liver, lung, pancreas, proventriculus, sciatic nerve, spleen, and thymus). Tissues were collected from 32-week-old commercial meat-type and Avian Disease and Oncology Laboratory experimental White Leghorn Line 0 chickens with the following different infection profiles: tV + A-, included in ovo-tolerized viraemic chickens with no neutralizing antibodies (NAbs) on any sampling; ntV + A-, included chickens that were viraemic and NAb-negative at the time of termination at 32 weeks post hatch, but had NAbs on up to two occasions; V+ A+, included chickens that were viraemic and NAb-positive at the time of termination at 32 weeks post hatch, and had NAbs on more than two occasions; V - A+, included chickens that were negative for viraemia and NAb-positive at the time of termination at 32 weeks post hatch, and had antibody on more than two occasions; V - A-, included chickens that were never exposed to ALV J virus. There was a direct correlation between viraemia and tissue distribution of gp85, regardless of the NAb status and strain of chickens, as expression of ALV J gp85 was noted in only viraemic chickens (tV + A-, ntV + A-, V+ A+), but not in non-viraemic seroconverted chickens (V - A+). Of the four oligonucleotide primers pairs used in PCR to identify ALV J provirus, only one primer set termed H5/H7 was useful in demonstrating ALV J proviral DNA in the majority of the tissues tested from non-viraemic, antibody-positive chickens (V - A+). The results suggest that PCR using primer pair H5/H7 is more sensitive than immunohistochemistry in identifying ALV J in chickens that have been exposed to virus, but are not actively viraemic.
采用免疫组织化学和聚合酶链反应（PCR）分别检测不同组织（肾上腺、骨髓、性腺、心脏、肾脏、肝脏、肺、胰腺、腺胃、坐骨神经、脾脏和胸腺）中禽白血病病毒（ALV）J病毒抗原gp85和前病毒DNA的存在情况。从32周龄的商品肉用型鸡以及禽病与肿瘤学实验室的实验性白来航0系鸡中采集组织，这些鸡具有以下不同的感染情况：tV + A - ，包括在卵内耐受的病毒血症鸡，在任何采样时均无中和抗体（NAbs）；ntV + A - ，包括在孵化后32周处死时为病毒血症且NAb阴性，但曾有过两次及以上NAbs的鸡；V + A + ，包括在孵化后32周处死时为病毒血症且NAb阳性，且有过两次以上NAbs的鸡；V - A + ，包括在孵化后32周处死时病毒血症阴性且NAb阳性，且有过两次以上抗体的鸡；V - A - ，包括从未接触过ALV J病毒的鸡。无论鸡的NAb状态和品系如何，病毒血症与gp85的组织分布之间存在直接相关性，因为仅在病毒血症鸡（tV + A - 、ntV + A - 、V + A + ）中检测到ALV J gp85的表达，而在非病毒血症的血清转化鸡（V - A + ）中未检测到。在用于PCR鉴定ALV J前病毒的四对寡核苷酸引物中，只有一对名为H5/H7的引物可用于在大多数来自非病毒血症、抗体阳性鸡（V - A + ）的测试组织中证明ALV J前病毒DNA的存在。结果表明，在鉴定已接触病毒但无活跃病毒血症的鸡中的ALV J时，使用引物对H5/H7的PCR比免疫组织化学更敏感。
