Masuki Kouhei, Nomura Yuji, Bhawal Ujjal Kumar, Sawajiri Masahiko, Hirata Isao, Nahara Yukinori, Okazaki Masayuki
Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University, Hiroshima, Japan.
Dent Mater J. 2007 Nov;26(6):861-9. doi: 10.4012/dmj.26.861.
The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators--namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)--on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 microM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G0/G1, S, G2/M phases). All four dental resin polymerization initiators induced G0/G1 cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.
本研究的目的是检测四种牙科树脂聚合引发剂，即过氧化苯甲酰（BPO）、樟脑醌（CQ）、甲基丙烯酸二甲氨基乙酯（DMAEMA）和二甲基对甲苯胺（DMPT），对人牙龈成纤维细胞（HGF）的凋亡和坏死影响。为此，使用细胞计数试剂盒-8评估1 mM BPO、CQ和DMAEMA以及500 microM DMPT对HGF细胞的生长抑制作用。然后，通过流式细胞术进行细胞周期分析，以评估碘化丙啶染色的细胞（细胞在G0/G1、S、G2/M期的分布）。所有四种牙科树脂聚合引发剂均诱导G0/G1期细胞周期停滞。至于细胞死亡模式（坏死和/或凋亡），则使用膜联蛋白V-异硫氰酸荧光素/碘化丙啶染色通过流式细胞术进行分析。所有四种牙科树脂聚合引发剂很可能诱导坏死。
Zotero 插件