Tang S X, Tayo G O, Tan Z L, Sun Z H, Shen L X, Zhou C S, Xiao W J, Ren G P, Han X F, Shen S B
Key Laboratory of Subtropical Agro-ecological Engineering, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, PR China.
J Anim Sci. 2008 May;86(5):1164-72. doi: 10.2527/jas.2007-0438. Epub 2008 Jan 18.
The effects of yeast culture and fibrolytic enzyme preparation (containing cellulase and xylanase) on in vitro fermentation characteristics of rice straw, wheat straw, maize stover, and maize stover silage were examined using an in vitro gas production technique. Four levels of yeast culture and fibrolytic enzyme supplements (0, 2.5, 5.0, and 7.5 g/kg of straw DM, respectively) were tested in a 4 x 4 factorial arrangement. Supplementation of yeast culture increased the cumulative gas production, theoretical maximum of gas production, rate of gas production, IVDMD, and in vitro OM disappearance (IVOMD), and decreased the lag time for each type of straw. Fibrolytic enzyme supplementation tended to increase cumulative gas production, theoretical maximum of gas production, and rate of gas production; prolonged lag time of gas production; and enhanced IVDMD and IVOMD for 4 types of cereal straws, with the significance of this effect being dependent on the level of supplemented enzymes. There were significant interactions between fibrolytic enzymes and yeast on all in vitro gas production parameters, IVDMD, and IVOMD of each type of straw. The outcome of this research indicated that the application of fibrolytic enzyme preparation and yeast culture could improve in vitro gas production fermentation of cereal straws.
采用体外产气技术，研究了酵母培养物和纤维分解酶制剂（含有纤维素酶和木聚糖酶）对稻草、小麦秸秆、玉米秸秆和玉米秸秆青贮体外发酵特性的影响。以4×4析因设计分别测试了4个水平的酵母培养物和纤维分解酶补充剂（分别为0、2.5、5.0和7.5 g/kg秸秆干物质）。添加酵母培养物可提高每种秸秆的累积产气量、理论最大产气量、产气速率、体外干物质消化率（IVDMD）和体外有机物消失率（IVOMD），并缩短滞后期。添加纤维分解酶倾向于提高累积产气量、理论最大产气量和产气速率；延长产气滞后期；提高4种谷物秸秆的IVDMD和IVOMD，这种效果的显著性取决于添加酶的水平。在每种秸秆的所有体外产气参数、IVDMD和IVOMD方面，纤维分解酶和酵母之间存在显著的交互作用。本研究结果表明，应用纤维分解酶制剂和酵母培养物可改善谷物秸秆的体外产气发酵。
