Chavalitshewinkoon P, Wilairat P
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1991 Dec;22(4):544-7.
The large scale in vitro cultivation method of Fairlamb et al (1985) was modified to contain human plasma-supplemented medium in HEPES buffer. After 4 days with no change of medium nor agitation of the culture flask, 27- to 50-fold increase in the starting parasitemia of trophozoites were obtained.
Fairlamb等人(1985年)的大规模体外培养方法经过改良,在HEPES缓冲液中加入了含人血浆的培养基。在培养基未更换且培养瓶未振荡的情况下培养4天后,滋养体的初始寄生虫血症增加了27至50倍。