Domracheva A G, Lebed' E S, Vladimirov A V, Bartoshevich Iu E
Antibiotiki. 1976 Jun;21(6):501-7.
High mutagenic activity of N-nitrozodimethylurea (NDMU), an agent of the group of the nitrozo compounds not studied in detail was shown with respect to prototrophic and auxotrophic strains of Actinomyces rimosus, an organism producing oxytetracycline and Penicillium chrysogenum, an organism producing penicillin. The rate of direct and back mutations in the auxotrophic strain of Act. rimosus under the effect of NDMU was many times higher than that of spontaneous mutations. NDMU was used at one of the selection stages at which a more active variant of Act. rimosus was obtained. This is evident of a possible use of the mutagen for induction of variation with respect to the quantitative feature of oxytetracycline production. A great number of morphologically changed forms and biochemical mutants of Pen. chrysogenum formed under the effect of this substance. NDMU induced a mutant of Pen. chrysogenum capable of selective synthesis of 6-aminopenicillinic acid without addition of the precursor.
N-亚硝基二甲脲（NDMU）是一类未被详细研究的亚硝基化合物，研究表明其对龟裂链霉菌（一种生产土霉素的微生物）的原养型和营养缺陷型菌株以及产黄青霉（一种生产青霉素的微生物）具有高诱变活性。在NDMU作用下，龟裂链霉菌营养缺陷型菌株的正向和回复突变率比自发突变率高很多倍。在获得更具活性的龟裂链霉菌变体的一个选择阶段使用了NDMU。这表明该诱变剂可能用于诱导土霉素产量定量特征的变异。在该物质作用下形成了大量产黄青霉形态改变的形式和生化突变体。NDMU诱导出一种产黄青霉突变体，该突变体无需添加前体就能选择性合成6-氨基青霉烷酸。
