Gruzina V D, Rusinov S F
Antibiotiki. 1983 Sep;28(9):647-9.
Survival and variation of strain 9741 of P. chrysogenum treated with N-nitroso-N-methyl biuret (NMB) at various stages of conidia initiation were studied. It was shown that the interval between the minimum and maximum levels of the conidia sensitivity to NMB was equal to 80 minutes, which corresponded to the period of DNA replication in the culture. When preliminary incubated conidia were subjected to the treatment, the frequency of the variants with higher potency increased as compared to the treatment of air-dried conidia. The variation coefficient increased, when conidia were treated with the mutagen after preliminary incubation for 80 or 100 minutes. This may be used for increasing the efficacy of directed improvement of the culture.
研究了产黄青霉9741菌株在分生孢子形成不同阶段用N-亚硝基-N-甲基缩二脲（NMB）处理后的存活情况和变异情况。结果表明，分生孢子对NMB的敏感性最低水平与最高水平之间的间隔为80分钟，这与培养物中DNA复制的时期相对应。当对预先培养的分生孢子进行处理时，与风干分生孢子的处理相比，效价较高的变异体频率增加。当分生孢子在预先培养80或100分钟后用诱变剂处理时，变异系数增加。这可用于提高定向改良培养物的效率。
