Kastenmayer Robin J, Perdue Kathy A, Elkins William R
National Institute of Allergy and Infectious Diseases, Comparative Medicine Branch, National Institutes of Health, Bethesda, MD, USA.
J Am Assoc Lab Anim Sci. 2008 Jan;47(1):26-30.
Murine norovirus (MNV) is a common viral infection of mice in many research facilities. MNV infects hematopoietic cells and alters their cellular morphology. Because of MNV's probable effects on the systemic immune response of infected mice the decision was made to eradicate the virus from 2 rooms containing infected animals in our vivarium. Two different eradication methods were selected. One room, in which most of the indirectly exposed sentinels had antibodies to MNV, was depopulated and thoroughly cleaned prior to repopulation. In the other room, in which only 13% of the sentinels had positive MNV titers, selective testing was used, and MNV-positive animals were removed. Data from surveillance of the sentinel mice exposed to dirty bedding indicate that the test-and-removal method was ineffective in eliminating MNV from the room, whereas sentinel mice in the room that underwent depopulation and cleaning prior to repopulation have not shown any evidence of MNV since December 2006.
小鼠诺如病毒（MNV）是许多研究机构中常见的小鼠病毒感染源。MNV感染造血细胞并改变其细胞形态。由于MNV可能会对受感染小鼠的全身免疫反应产生影响，因此我们决定在动物饲养室内，从两个饲养着受感染动物的房间中根除该病毒。我们选择了两种不同的根除方法。其中一个房间里，大多数间接接触的哨兵小鼠都有针对MNV的抗体，在重新饲养之前，该房间被清空并彻底清洁。另一个房间里，只有13%的哨兵小鼠MNV滴度呈阳性，我们采用了选择性检测，并移除了MNV呈阳性的动物。对接触脏垫料的哨兵小鼠进行监测的数据表明，检测和移除方法在从房间中清除MNV方面无效，而在重新饲养之前进行了种群清空和清洁的房间里，自2006年12月以来，哨兵小鼠没有出现任何MNV感染的迹象。
