Rodrigues Daniele Masselli, Moreira Josélia Cristina de Oliveira, Lancellotti Marcelo, Gilioli Rovilson, Corat Marcus Alexandre Finzi
Multidisciplinary Center for Biological Research on Laboratory Animal Science (CEMIB), Animal Health Laboratory, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
Department of Biochemistry, Institute of Biology, University of Campinas, Campinas, São Paulo 13083-970, Brazil.
Exp Anim. 2017 May 3;66(2):115-124. doi: 10.1538/expanim.16-0027. Epub 2016 Dec 29.
Murine norovirus (MNV) is a single-stranded positive-sense RNA virus of the Caliciviridae family. MNV has been reported to infect laboratory mice with the ability to cause lethal infections in strains lacking components of the innate immune response. Currently, MNV is considered the most prevalent infectious agent detected in laboratory mouse facilities. In this study, mice in 22 laboratory animal facilities within Brazil were analyzed for MNV infection. Using primers targeting a conserved region of the viral capsid, MNV was detected by RT-PCR in 137 of 359 mice from all 22 facilities. Nucleotide sequencing and phylogenetic analysis of the capsid region from the viral genome showed identity ranging from 87% to 99% when compared to reported MNV sequences. In addition, RAW264.7 cells inoculated with a mouse fecal suspension displayed cytopathic effect after the fifth passage. This study represents the first report of MNV in mouse colonies in Brazilian laboratory animal facilities, emphasizing the relevance of a health surveillance program in such environments.
小鼠诺如病毒（MNV）是杯状病毒科的一种单链正义RNA病毒。据报道，MNV能够感染实验室小鼠，并在缺乏先天免疫反应成分的品系中引发致命感染。目前，MNV被认为是在实验室小鼠设施中检测到的最普遍的传染因子。在本研究中，对巴西22个实验动物设施中的小鼠进行了MNV感染分析。使用靶向病毒衣壳保守区域的引物，通过RT-PCR在来自所有22个设施的359只小鼠中的137只中检测到了MNV。对病毒基因组衣壳区域的核苷酸测序和系统发育分析表明，与已报道的MNV序列相比，其同一性范围为87%至99%。此外，接种小鼠粪便悬液的RAW264.7细胞在传代五次后出现细胞病变效应。本研究是巴西实验动物设施中小鼠群体中MNV的首次报道，强调了在此类环境中开展健康监测计划的重要性。
