一种用于定量测定人体呼出气体中碳氢化合物的系统。

A system for the quantitative determination of hydrocarbons in human breath.

作者信息

Seabra L, Braganza J M, Jones M F

机构信息

Department of Gastroenterology, Manchester Royal Infirmary, UK.

出版信息

J Pharm Biomed Anal. 1991;9(9):693-7. doi: 10.1016/0731-7085(91)80208-q.

DOI:10.1016/0731-7085(91)80208-q

PMID:1821144

Abstract

A method for the analysis of hydrocarbons in exhaled human breath samples has been developed and its quantitative performance optimized and exhaustively validated. The method involves preconcentration on a solid absorbent at 0 degree C and desorption at 250 degrees C to a packed column gas chromatograph. Calibrations for ethane and pentane are reproducible and linear over the concentration ranges found in human breath samples. The technique is now available for study of conditions, such as cystic fibrosis, in which an oxidative stress component in tissue injury is suspected.

摘要

一种用于分析人体呼出气体样本中碳氢化合物的方法已经开发出来，其定量性能得到了优化并经过了全面验证。该方法包括在0摄氏度下于固体吸附剂上进行预浓缩，并在250摄氏度下解吸至填充柱气相色谱仪中。乙烷和戊烷的校准在人体呼出气体样本中发现的浓度范围内具有可重复性和线性。该技术现在可用于研究诸如囊性纤维化等疾病，在这些疾病中怀疑组织损伤存在氧化应激成分。