Slabas A R, Walker D A
Biochem J. 1976 Mar 1;153(3):613-9. doi: 10.1042/bj1530613.
Spinach chloroplast phosphoribulokinase is inhibited by DL-glyceraldehyde. The inhibition is non-competitive with respect to ribulose 5-phosphate (Ki 19mM) and ATP (Ki 20mM). The inhibition is discussed in relation to a previously reported inhibition of CO2 assimilation in intact and envelope-free chloroplasts by DL-glyceraldehyde. It is concluded that the inhibition of phosphoribulokinase is insufficient to account for the inhibition, by DL-glyceraldehyde, of O2 evolution with ribose 5-phosphate as substrate and that a further site of inhibition is also present in this system.
菠菜叶绿体磷酸核酮糖激酶受DL-甘油醛抑制。这种抑制作用对于5-磷酸核酮糖(Ki为19mM)和ATP(Ki为20mM)而言是非竞争性的。结合之前报道的DL-甘油醛对完整叶绿体和无包膜叶绿体中CO2同化作用的抑制,对该抑制作用进行了讨论。得出的结论是,磷酸核酮糖激酶的抑制不足以解释DL-甘油醛对以5-磷酸核糖为底物时氧气释放的抑制作用,并且该系统中还存在另一个抑制位点。