Deng Yu, Liu En-Mei, Zhao Xiao-Dong, Ding Yuan, Li Qu-Bei, Luo Zheng-Xiu, Wang Li-Jia, Huang Ying, Yang Xi-Qiang
Divison of Respiratory Disorder, Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
Zhonghua Er Ke Za Zhi. 2007 Oct;45(10):732-5.
The impact of human bocavirus (HBoV), a newly identified human parvovirus, on childhood persistent wheezing has not been identified. In this study, the clinical features of infantile persistent wheezing induced by HBoV was analyzed.
Tracheal aspirates were collected by bronchofibroscope or nasopharyngeal (NP) aspirates from April, 2006 to January, 2007. HBoV DNA in the tracheal aspirates of 33 children with persistent wheezing and in NP aspirates of 6 children with persistent wheezing, who had at least or more than four weeks wheezing. RSV was identified by virus isolation in Hep-2 cells and antigen detetion by direct immunofluorescence assay (DIFA) which was also used for diagnosis of adenovirus, influenza A and B, parainfluenza 1, 2, 3 infection.
Of the 39 children with persistent wheezing, 12 cases (31%) were positive for HBoV DNA. Age of HBoV-positive patients ranged from 2 month to 1 year. The results of sequencing of PCR products proved that sequences of HBoV DNA from these 12 samples were exactly identical to the those of HBoV stored in GeneBank (accession numbers DQ000495 and DQ000496). Two cases with HBoV infection were found to be co-infected with RSV. Ten of the 12 HBoV-positive samples were collected during the period from winter to spring (1 in November, 4 in December, 2 in January and 3 in April), the other two HBoV-positive samples were collected during the period from summer to autumn (1 in May and the other in July). Seven of the 12 HBoV DNA-positive patients had fever, 5 of them had high fever. Significantly more patients with HBoV infection had fever as compared to patients with RSV infection. All the HBoV positive patients showed abnormal findings on chest X ray such as interstitial infiltrates, lung infiltration and hyperinflation. Abnormal findings on chest X ray were found in higher proportion of HBoV positive patients as compared with RSV positive patients. And other manifestations such as wheezing, cough and respiratory distress had no significant difference between HBoV and RSV infected patients.
This study further demonstrated that HBoV probably is a common pathogen of lower respiratory infection in children and might particularly be associated with persistent wheezing.
新型人类细小病毒——人博卡病毒（HBoV）对儿童持续性喘息的影响尚未明确。本研究分析了由HBoV引起的婴儿持续性喘息的临床特征。
于2006年4月至2007年1月期间，通过支气管纤维镜收集气管吸出物或鼻咽（NP）吸出物。收集了33例持续性喘息儿童的气管吸出物以及6例持续性喘息儿童的NP吸出物，这些儿童喘息至少持续四周或更长时间。呼吸道合胞病毒（RSV）通过在Hep-2细胞中进行病毒分离以及采用直接免疫荧光测定法（DIFA）检测抗原进行鉴定，DIFA也用于诊断腺病毒、甲型和乙型流感病毒、副流感病毒1、2、3感染。
在39例持续性喘息儿童中，12例（31%）HBoV DNA检测呈阳性。HBoV阳性患者的年龄范围为2个月至1岁。PCR产物测序结果证明，这12个样本中的HBoV DNA序列与基因库中存储的HBoV序列（登录号DQ000495和DQ000496）完全相同。发现2例HBoV感染患儿同时感染了RSV。12例HBoV阳性样本中有10例是在冬春季节收集的（1例在11月，4例在12月，2例在1月，3例在4月），另外2例HBoV阳性样本是在夏秋季节收集的（1例在5月，另1例在7月）。12例HBoV DNA阳性患者中有7例发热，其中5例为高热。与RSV感染患者相比，HBoV感染患者发热的比例明显更高。所有HBoV阳性患者胸部X线检查均有异常表现，如间质浸润、肺部浸润和肺过度充气。与RSV阳性患者相比，HBoV阳性患者胸部X线异常表现的比例更高。而喘息、咳嗽和呼吸窘迫等其他表现，在HBoV感染患者和RSV感染患者之间无显著差异。
本研究进一步证明，HBoV可能是儿童下呼吸道感染的常见病原体，尤其可能与持续性喘息有关。
