Hayatsu Norihito, Ogasawara Satoshi, Kaneko Mika Kato, Kato Yukinari, Narimatsu Hisashi
Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8568, Japan.
Biochem Biophys Res Commun. 2008 Apr 4;368(2):217-22. doi: 10.1016/j.bbrc.2008.01.058. Epub 2008 Jan 22.
Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.
硫酸角质素（KS）蛋白聚糖在许多组织的细胞外基质或细胞表面表达，主要存在于角膜、软骨和脑组织中。然而，其结构、功能和调控机制仍知之甚少。我们使用抗KS抗体（5D4）通过蛋白质印迹法和流式细胞术对胶质母细胞瘤细胞系中的KS表达进行研究，结果显示LN229胶质母细胞瘤细胞在细胞表面高表达KS。实时定量PCR分析表明，LN229表达高水平的硫酸角质素Gal-6-硫酸转移酶。本研究结果还表明，LN229可产生重组5D4反应性聚集蛋白聚糖。综上所述，这些结果表明LN229可产生5D4反应性高硫酸化KS，有助于研究胶质母细胞瘤中KS的结构和功能。
