Wood H N, Pomerantz A H, Binns A N, Allfrey V G, Braun A C
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4301-5. doi: 10.1073/pnas.75.9.4301.
A cyclic AMP-like substance has been isolated from higher plant tissues which can be quantitated with the use of a radioimmunoassay similar to that described by A. L. Steiner, D. M. Kipnis, R. Utiger, and C. Parker [(1969) Proc. Natl. Acad. Sci. USA 64, 367-373]. This compound has been extensively purified and is chromatographically distinct from authentic cyclic AMP. This cyclic AMP-like compound inhibited beef heart 3':5'-cyclic-nucleotide phosphodietsterase (3':5'-cyclic-nucleotide 5'-nucleotidohydrolase, EC 3.1.4.17), with half-maximal inhibition occurring at a concentration of 7.6 X 10(-10) M cyclic AMP equivalents. The compound also inhibited cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase; EC 2.7.1.37) from bovine heart, with half-maximal inhibition of mixed histone phosphorylation occurring at 8.0 X 10(-11) M cyclic AMP equivalents. Equipotent inhibition of phosphorylation and associated trace ATPase activity were observed with the purified catalytic subunit of cyclic AMP-dependent protein kinase from calf thymus with a synthetic heptapeptide as substrate. Moreover, steady-state kinetic analysis of this inhibition in the latter system showed it to be nonlinear and noncompetitive versus MgATP.
一种类似环磷酸腺苷（cAMP）的物质已从高等植物组织中分离出来，可使用类似于A. L. 施泰纳、D. M. 基普尼斯、R. 乌蒂格和C. 帕克所描述的放射免疫分析法进行定量分析[（1969年）《美国国家科学院院刊》64卷，第367 - 373页]。该化合物已被广泛纯化，在色谱上与纯的环磷酸腺苷不同。这种类似环磷酸腺苷的化合物抑制牛心3':5'-环核苷酸磷酸二酯酶（3':5'-环核苷酸5'-核苷酸水解酶，EC 3.1.4.17），在相当于7.6×10⁻¹⁰ M环磷酸腺苷的浓度下出现半数最大抑制。该化合物还抑制来自牛心的环磷酸腺苷依赖性蛋白激酶（ATP：蛋白磷酸转移酶；EC 2.7.1.37），在相当于8.0×10⁻¹¹ M环磷酸腺苷的浓度下出现对混合组蛋白磷酸化的半数最大抑制。以合成七肽为底物时，观察到小牛胸腺中环磷酸腺苷依赖性蛋白激酶的纯化催化亚基对磷酸化及相关微量ATP酶活性有等效抑制作用。此外，在后者系统中对这种抑制作用的稳态动力学分析表明，它对MgATP呈非线性和非竞争性。
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