Wang Zhiping, Zhang Shicui, Wang Guangfeng, An Yan
Department of Marine Biology, Ocean University of China, Qingdao, People's Republic of China.
PLoS One. 2008 Jan 23;3(1):e1463. doi: 10.1371/journal.pone.0001463.
Most fish embryos that develop externally are exposed to an environment full of microbes. How they survive microbial attacks are not understood to date. Here we demonstrated that the egg cytosol prepared from the newly fertilized eggs of zebrafish Danio rerio is capable of killing the Gram-negative bacterium Escherichia coli, via in vitro assay system of the complement activity established. All findings indicate that it is the complement system operating via the alternative pathway that is attributable to the bacteriolytic activity. This is the first report providing the evidence for the functional role of the maternal complement components in fish eggs, paving the way for study of maternal immunity in other organisms whose eggs are fertilized in vitro.
大多数在体外发育的鱼类胚胎都暴露在充满微生物的环境中。迄今为止，它们如何在微生物攻击下存活尚不清楚。在这里，我们通过建立的补体活性体外检测系统证明，从斑马鱼（Danio rerio）新受精卵制备的卵细胞质能够杀死革兰氏阴性菌大肠杆菌。所有研究结果表明，通过替代途径发挥作用的补体系统具有溶菌活性。这是第一份为鱼卵中母体补体成分的功能作用提供证据的报告，为研究其他体外受精生物的母体免疫铺平了道路。
