Swain P, Dash S, Bal J, Routray P, Sahoo P K, Sahoo S K, Saurabh S, Gupta S D, Meher P K
Aquatic Animal Health Division, Central Institute of Freshwater Aquaculture, Kausalyaganga-751002, Bhubaneswar, Orissa, India.
Fish Shellfish Immunol. 2006 Apr;20(4):519-27. doi: 10.1016/j.fsi.2005.06.011. Epub 2005 Sep 12.
Lack of immune competence in the early stages of life leads to severe mortality in larval stages of different fish species including Indian major carp (IMC). Investigation through indirect enzyme linked immunosorbent assay (ELISA) and agglutination test revealed a significant increase in specific serum antibody response in the brood fish of Indian major carp, Labeo rohita (Ham.) following immunisation with a virulent Aeromonas hydrophila bacterin 1 month prior to breeding, which was transferred to larvae through the egg. No significant differences (P > 0.05) in mean antibody levels in larvae at the 1st and 2nd weeks post-hatch was recorded while a slight rise in antibody level was observed in 3-week-old fry, perhaps due to exposure to A. hydrophila present in the aquatic environment. Immunised brood fish serum, egg and larval extracts in non-reducing sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and subsequent western blot analysis revealed an antibody molecule of approximate molecular weight 210 kDa. On challenge with virulent A. hydrophila, a significant reduction in mortality was recorded in immunised larvae and fry (58.0, 43.75 and 37.14% in the 1st, 2nd and 3rd week, respectively) relative to control fish (87.0, 79.0 and 76.4% in 1st, 2nd and 3rd week, respectively). The present study indicated the role of maternally derived antibody in protection of hatchlings of Indian major carp against specific pathogens.
生命早期阶段缺乏免疫能力会导致包括印度主要鲤鱼（IMC）在内的不同鱼类幼体阶段出现严重死亡。通过间接酶联免疫吸附测定（ELISA）和凝集试验进行的调查显示，在繁殖前1个月用强毒嗜水气单胞菌菌苗对印度主要鲤鱼露斯塔野鲮（Ham.）的亲鱼进行免疫后，其特异性血清抗体反应显著增加，这种抗体通过卵传递给幼体。在孵化后第1周和第2周的幼体中，平均抗体水平没有显著差异（P>0.05），而在3周龄的鱼苗中观察到抗体水平略有上升，这可能是由于接触了水环境中存在的嗜水气单胞菌。在非还原十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）及随后的蛋白质印迹分析中，免疫亲鱼血清、卵和幼体提取物显示出一种分子量约为210 kDa的抗体分子。在用强毒嗜水气单胞菌攻击后，免疫幼体和鱼苗的死亡率显著降低（第1周、第2周和第3周分别为58.0%、43.75%和37.14%），相对于对照鱼（第1周、第2周和第3周分别为87.0%、79.0%和76.4%）。本研究表明母源抗体在保护印度主要鲤鱼幼体免受特定病原体侵害方面的作用。
