Ardesjö Brita, Portela-Gomes Guida M, Rorsman Fredrik, Gerdin Eva, Lööf Lars, Grimelius Lars, Kämpe Olle, Ekwall Olov
Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Inflamm Bowel Dis. 2008 May;14(5):652-61. doi: 10.1002/ibd.20370.
A number of autoantibodies have been reported in inflammatory bowel disease (IBD). The aim of this study was to investigate to what extent sera from patients with IBD contain autoantibodies directed against normal human gastrointestinal mucosa.
Samples of sera from 50 patients with IBD and 50 healthy subjects were used for immunostaining of normal and affected human gastrointestinal tissues.
Eighty-four percent of the sera from IBD patients showed immunoreactivity against goblet cells in the appendix compared with 8% of the sera from healthy subjects. Goblet cell reactivity of IBD patient sera varied between regions in the gastrointestinal tract. Sera from healthy subjects only reacted with goblet cells in the appendix. In the colon and the appendix, goblet cell reactivity of IBD sera was generally weak at the base of the crypts and gradually increased toward the lumen. Three IBD sera samples reacted with gastrin cells in the antrum. In colon biopsies from patients with ulcerative colitis, immunoreactivity against the remaining goblet cells showed an inverse correlation with inflammatory activity.
These findings suggest that immunoreactivity against goblet cells may be of central importance in the pathogenesis of IBD. Identification of goblet cell antigens could lead to a better understanding of IBD and provide a new diagnostic tool.
炎症性肠病（IBD）中已报道了多种自身抗体。本研究旨在调查IBD患者血清中针对正常人胃肠道黏膜的自身抗体的存在程度。
采用50例IBD患者和50例健康受试者的血清样本，对正常和病变的人体胃肠道组织进行免疫染色。
IBD患者血清中84%对阑尾杯状细胞显示免疫反应性，而健康受试者血清中这一比例为8%。IBD患者血清对杯状细胞的反应性在胃肠道不同区域有所不同。健康受试者的血清仅与阑尾中的杯状细胞发生反应。在结肠和阑尾中，IBD血清对杯状细胞的反应性在隐窝底部通常较弱，并向管腔逐渐增强。3份IBD血清样本与胃窦中的胃泌素细胞发生反应。在溃疡性结肠炎患者的结肠活检中，对其余杯状细胞的免疫反应性与炎症活动呈负相关。
这些发现表明，针对杯状细胞的免疫反应性可能在IBD发病机制中起核心作用。鉴定杯状细胞抗原可能有助于更好地理解IBD并提供一种新的诊断工具。
