Department of Pediatrics, Gastroenterology and Nutrition, Polish-American Children's Hospital, Jagiellonian University Medical College, 265 Wielicka Str., 30-663 Cracow, Poland.
World J Gastroenterol. 2009 Nov 14;15(42):5287-94. doi: 10.3748/wjg.15.5287.
To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease (IBD).
Sixty-one adolescents (mean age 15 years, SD+/-4.13) were included in the study. Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions. The number of microbes belonging to the same group was calculated per weight of collected tissue. The mucus thickness in frozen samples was measured under a fluorescent microscope.
The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases. Streptococcus spp. were predominant in the inflamed mucosa of Crohn's disease (CD) patients (80% of all bacteria), and Lactobacillus spp. were predominant in ulcerative colitis patients (90%). The differences were statistically significant (P=0.01-0.001). Lower number of bifidobacteria was observed in the whole IBD group. A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and, to a lesser extent, of Streptococcus in biopsies from CD patients. The mucus layer in the inflamed sites was significantly thinner as compared to controls (P=0.0033) and to non-inflamed areas in IBD patients (P=0.031).
The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases, their numbers decreased in relation to higher clinical and endoscopic activity of the disease.
评估炎症性肠病（IBD）青少年的黏膜相关细菌菌群和黏液层。
纳入 61 名青少年（平均年龄 15 岁，标准差+/-4.13）进行研究。对 IBD 患者的炎症和非炎症黏膜以及功能性腹痛对照者的肠道活检进行需氧和厌氧培养。根据采集组织的重量计算同组微生物的数量。在荧光显微镜下测量冷冻样本中的黏液厚度。
IBD 患者的炎症和非炎症黏膜中不同细菌群的比例因特定疾病而异。在克罗恩病（CD）患者的炎症黏膜中，链球菌属（Streptococcus spp.）占主导地位（所有细菌的 80%），而溃疡性结肠炎患者以乳杆菌属（Lactobacillus spp.）为主（90%）。差异具有统计学意义（P=0.01-0.001）。整个 IBD 组双歧杆菌的数量也较低。还发现 CD 患者的临床和内镜严重程度与活检中乳杆菌和链球菌数量减少有关，而链球菌的数量减少程度较小。与对照者（P=0.0033）和 IBD 患者的非炎症部位相比，炎症部位的黏液层明显更薄（P=0.031）。
IBD 患者的黏膜明显变薄，表现出某些特定疾病的优势需氧菌，其数量与疾病的临床和内镜活动度增加有关。
