Krzystek-Korpacka Malgorzata, Neubauer Katarzyna, Berdowska Izabela, Boehm Dorota, Zielinski Bogdan, Petryszyn Pawel, Terlecki Grzegorz, Paradowski Leszek, Gamian Andrzej
Department of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland.
Inflamm Bowel Dis. 2008 Jun;14(6):794-802. doi: 10.1002/ibd.20383.
Advanced oxidation protein products (AOPPs) are new protein markers of oxidative stress with pro-inflammatory properties, accumulated in many pathological conditions. The issue of their enhanced formation in IBD has not been addressed yet.
The concentration of relative AOPPs (rAOPP; concentration of AOPPs divided by albumin level) were measured in 68 subjects with ulcerative colitis (UC), 50 subjects with Crohn's disease (CD) and 45 healthy volunteers, and related to disease phenotype, clinical and biochemical activity, and therapeutic strategy. Diagnostic utility of rAOPP was evaluated by ROC analysis.
In comparison with controls (1.367 micromol/g), rAOPP were increased in inactive (1.778 micromol/g, P = 0.053) and active (1.895 micromol/g, P = 0.013) UC and in active (1.847 micromol/g, P = 0.003) CD. In CD, but not UC, rAOPP correlated with disease activity (r = 0.42, P = 0.013). Significant correlations with the inflammatory/malnutrition indices-erythrocyte sedimentation rate (ESR) (r = 0.53), leukocytes (r = 0.33), platelets (r = 0.38), IL-6 (r = 0.36), and transferrin (r = -0.35) were demonstrated in CD. In UC, rAOPP correlated only with ESR (r = 0.35) and IL-6 (r = 0.30). Instead, associations with antioxidant dismutase (r = 0.29) and catalase (r = 0.22) were observed. The diagnostic power of rAOPP in discriminating diseased from non-diseased subjects was less than that of C-reactive protein (CRP). Simultaneous determination of rAOPP and CRP did not significantly improve the power of single CRP determination.
IBD was associated with enhanced formation of AOPP, which differed between C and UC with respect to the relationship between rAOPP and disease activity, inflammatory and antioxidant response. These differences may reflect divergent ways that oxidative stress develops in CD and UC. The diagnostic power of rAOPP was insufficient for its clinical application.
晚期氧化蛋白产物（AOPPs）是具有促炎特性的氧化应激新蛋白标志物，在许多病理状况下会蓄积。IBD中其生成增加的问题尚未得到探讨。
测定了68例溃疡性结肠炎（UC）患者、50例克罗恩病（CD）患者和45名健康志愿者的相对AOPPs（rAOPP；AOPPs浓度除以白蛋白水平）浓度，并将其与疾病表型、临床和生化活性以及治疗策略相关联。通过ROC分析评估rAOPP的诊断效用。
与对照组（1.367微摩尔/克）相比，rAOPP在非活动期（1.778微摩尔/克，P = 0.053）和活动期（1.895微摩尔/克，P = 0.013）的UC患者以及活动期（1.847微摩尔/克，P = 0.003）的CD患者中升高。在CD患者中，而非UC患者中，rAOPP与疾病活动度相关（r = 0.42，P = 0.013）。在CD患者中显示出与炎症/营养不良指标——红细胞沉降率（ESR）（r = 0.53）、白细胞（r = 0.33）、血小板（r = 0.38）、IL-6（r = 0.36）和转铁蛋白（r = -0.35）有显著相关性。在UC患者中，rAOPP仅与ESR（r = 0.35）和IL-6（r = 0.30）相关。相反，观察到与抗氧化剂超氧化物歧化酶（r = 0.29）和过氧化氢酶（r = 0.22）有关联。rAOPP区分患病与未患病个体的诊断能力低于C反应蛋白（CRP）。同时测定rAOPP和CRP并未显著提高单一CRP测定的效能。
IBD与AOPP生成增加有关，在CD和UC中，rAOPP与疾病活动度、炎症和抗氧化反应之间的关系有所不同。这些差异可能反映了CD和UC中氧化应激发展的不同方式。rAOPP的诊断能力不足以用于临床应用。
