Unit of Biochemistry, Department of Biomedicine, Faculty of Medicine, University of Porto, Al. Prof. Hernâni Monteiro, 4200-319, Porto, Portugal.
Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Plant Foods Hum Nutr. 2022 Dec;77(4):560-567. doi: 10.1007/s11130-022-01003-z. Epub 2022 Aug 19.
Date seed is a by-product of Phoenix dactylifera L. fruit which is well recognized for its polyphenols content and numerous health-beneficial effects. Due to the increasing interest in natural phytochemicals with antioxidant activities, the present study aimed to extract polyphenols from both raw and roasted date seeds and investigate the anti-angiogenic effect of these two extracts (raw and roasted date seed polyphenols extracts (DSPE) at 25 and 50 µg/mL) using human microvascular endothelial cells (HMVEC). Our results showed that both raw and roasted DSPE suppressed some angiogenesis features in a dose-dependent manner including cell proliferation, migration, and capillary-like structure formation, of which raw DSPE was more potent inhibitor than roasted DSPE. Reduction in reactive oxygen species, as well as enhancement of superoxide dismutase activity occurred using both raw and roasted date seed polyphenols extracts. However, no changes were observed in advanced oxidation protein products versus control. Taken together, our data indicated that raw and roasted DSPE possess antioxidant activity, which suggested their potential use as a source of polyphenols with anti-angiogenic properties. Nevertheless, further studies are required to explore the underlying mechanisms responsible for their anti-angiogenic activities.
椰枣种子是椰枣果实的副产品，因其富含多酚类物质和多种有益健康的功效而广为人知。由于人们对具有抗氧化活性的天然植物化学物质的兴趣日益浓厚，本研究旨在从生椰枣种子和烤椰枣种子中提取多酚，并研究这两种提取物（生椰枣种子和烤椰枣种子多酚提取物（DSPE）在 25 和 50μg/ml）对人微血管内皮细胞（HMVEC）的抗血管生成作用。我们的结果表明，生椰枣和烤椰枣 DSPE 均以剂量依赖的方式抑制某些血管生成特征，包括细胞增殖、迁移和毛细血管样结构形成，其中生椰枣 DSPE 的抑制作用强于烤椰枣 DSPE。生椰枣和烤椰枣多酚提取物均可降低活性氧，增强超氧化物歧化酶活性。然而，与对照组相比，高级氧化蛋白产物没有变化。综上所述，我们的数据表明，生椰枣和烤椰枣 DSPE 具有抗氧化活性，这表明它们可能被用作具有抗血管生成特性的多酚来源。然而，需要进一步研究来探索其抗血管生成活性的潜在机制。
