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[核仁蛋白的可能细胞光度测定法。核仁装置状态的研究前景]

[The possible cytophotometry of nucleolar proteins. The prospects for research on the status of the nucleolus apparatus].

作者信息

Brodskiĭ V Ia, Marshak T L, Avrushchenko M Sh, Dungenova R E

出版信息

Tsitologiia. 1991;33(8):65-74.

PMID:1821499
Abstract

Possibility of cytophotometry application was established for the acid nucleolar proteins reacting with silver nitrate. The increase in transcription in the Purkinje neurons results in the expansion of the Ag-proteins areas, whereas their amount may be not increased. The amount and area of the Ag-proteins double in hepatocytes through polyploidization, but the number of nucleoli does not correspond to the gene dosage. This lack of correspondence was also revealed in micronucleoli. The number of nucleoli is not similar in the nuclei of some binuclear hepatocytes, and this disproportionality increases through polyploidization.

摘要

已确定细胞光度测定法可用于与硝酸银反应的酸性核仁蛋白。浦肯野神经元中转录的增加导致银蛋白区域的扩大,但其数量可能并未增加。通过多倍体化,肝细胞中银蛋白的数量和面积增加了一倍,但核仁数量与基因剂量并不对应。这种不对应在微核仁中也有体现。一些双核肝细胞的细胞核中核仁数量并不相同,且这种不均衡通过多倍体化而加剧。

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