Naskalski J W, Kusnierz-Cabala B, Kedra B, Dumnicka P, Panek J, Maziarz B
Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland.
Adv Med Sci. 2007;52:129-34.
The relationship between direct count of peripheral blood leucocyte populations and plasma concentrations of IL-6, IL-8, sTNFR-55 and sTNFR-75 during five initial days of acute pancreatitis was studied.
Most significant relationship was found for monocytes, which correlated with sTNFR-55 (R = 0.38, p < 0.05) and sTNFR-75 (R = 0.41, p < 0.05 and R = 0.55, p < 0.01 during 1st and 2nd day, respectively). Later, in days 2, 3 and 4 an interrelation between monocytes and IL-6 (R = 0.49 to R = 0.41, p < 0.01) was observed. Monocytes also correlated with IL-8 in days 2 and 3 (R = 0.41, p < 0.05 and R = 0.43, p < 0.01, respectively). Neutrophil count correlated with IL-6 in days 3 and 4 (R = 0.34, p < 0.05 and R = 0.56, p < 0.01, respectively) and with IL-8 in the 4th day only (R = 0.39, p < 0.05). No significant correlations of lymphocyte, eosinophil and basophil direct counts with cytokines and receptors during the initial 5 days of AP were found.
Observed relationships between monocyte direct counts and plasma cytokine levels reflect monocytes involvement in the development of acute pancreatitis.
研究了急性胰腺炎发病最初5天外周血白细胞群体直接计数与白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、可溶性肿瘤坏死因子受体55（sTNFR-55）和可溶性肿瘤坏死因子受体75（sTNFR-75）血浆浓度之间的关系。
发现单核细胞的相关性最为显著，其与sTNFR-55相关（R = 0.38，p < 0.05），与sTNFR-75相关（R = 0.41，p < 0.05；在第1天和第2天分别为R = 0.55，p < 0.01）。随后，在第2、3和4天观察到单核细胞与IL-6之间存在相互关系（R = 0.49至R = 0.41，p < 0.01）。在第2天和第3天，单核细胞也与IL-8相关（分别为R = 0.41，p < 0.05；R = 0.43，p < 0.01）。中性粒细胞计数在第3天和第4天与IL-6相关（分别为R = 0.34，p < 0.05；R = 0.56，p < 0.01），仅在第4天与IL-8相关（R = 0.39，p < 0.05）。在急性胰腺炎发病最初5天，未发现淋巴细胞、嗜酸性粒细胞和嗜碱性粒细胞直接计数与细胞因子及受体之间存在显著相关性。
观察到的单核细胞直接计数与血浆细胞因子水平之间的关系反映了单核细胞参与急性胰腺炎的发生发展。
