Department of Emergency, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
School of Basic Medicine, Anhui Medical University, Hefei 230032, China.
Molecules. 2022 Jun 17;27(12):3880. doi: 10.3390/molecules27123880.
Acute pancreatitis (AP) is a pancreatic inflammatory disease that varies greatly in course and severity. To further the understanding of the pathology of AP, we carried out data-independent acquisition-based proteomic analyses using proteins extracted from the plasma of patients with severe acute pancreatitis (SAP) (experimental group) and healthy volunteers (control group). Compared to the control group, there were 35 differentially expressed proteins (DEPs) in the plasma of patients with SAP. Of those, the expression levels for 6 proteins were significantly increased, and 29 proteins were significantly decreased. Moreover, six candidate biomarkers-VWF, ORM2, CD5L, CAT, IGLV3-10, and LTF-were matched as candidate biomarkers of the disease severity of AP. The area under the receiver operating characteristic of 0.903 (95% CI: 0.839, 0.967) indicated that this combination of these six candidate biomarkers had a good prediction accuracy for predicting the severity of AP. Our study provides specific DEPs that may be useful in the diagnosis and prognosis of SAP, which suggests new theoretical bases for the occurrence and development of SAP and offers potential novel treatment strategies for SAP.
急性胰腺炎（AP）是一种胰腺炎症性疾病，其病程和严重程度差异很大。为了进一步了解 AP 的病理学，我们使用从重症急性胰腺炎（SAP）患者（实验组）和健康志愿者（对照组）的血浆中提取的蛋白质进行了基于数据非依赖采集的蛋白质组学分析。与对照组相比，SAP 患者血浆中有 35 种差异表达蛋白（DEPs）。其中，6 种蛋白的表达水平显著升高，29 种蛋白显著降低。此外，还匹配了 6 种候选生物标志物-VWF、ORM2、CD5L、CAT、IGLV3-10 和 LTF-作为 AP 疾病严重程度的候选生物标志物。ROC 曲线下面积为 0.903（95%CI：0.839，0.967），表明这六种候选生物标志物的组合对预测 AP 的严重程度具有良好的预测准确性。我们的研究提供了可能有助于 SAP 诊断和预后的特定 DEPs，这为 SAP 的发生和发展提供了新的理论基础，并为 SAP 提供了潜在的新的治疗策略。
