Jabłońska E, Kiersnowska-Rogowska B, Ratajczak W, Rogowski F, Sawicka-Powierza J
Department of Immunology, Medical University of Białystok, ul. Waszyngtona 15A, 15-274 Białystok, Poland.
Adv Med Sci. 2007;52:154-8.
The study objective was to investigate the production of NO, cGMP and superoxide anion radical by neutrophils, and to examine alterations in serum or plasma total nitric oxide, MDA and cGMP levels in B-CLL patients.
PMNs were isolated from 20 patients with B-CLL. Total nitrite was measured in cell supernatants and serum by Griess method. The generation of superoxide anion radical by cells was estimated using cytochrom-c reduction test. The cGMP level in cell supernatants and plasma was assessed by ELISA kit whereas serum MDA level using a spectrophotometric assay by Guege and Aus.
The PMNs in B-CLL patients were characterised by impaired NO generation and enhanced cGMP production. Contrary to the control group, no significant effect was found of rhlL-15 and rhIL-18 on the release of these mediators by PMNs. Superoxide anion radical release by PMNs was decrease. Serum MDA and plasma cGMP levels were elevated in B-CLL patients as compared to the controls.
The reduced production of nitric oxide and superoxide anion radicals by PMNs in B-CLL may impair the cytotoxic effect of neutrophils on leukaemic B cells. Low secretion of nitric oxide by PMNs and high levels of cGMP in PMN supernatants suggest that activation of guanyl cyclase (sGC) in these patients may occur in the presence of agents other than nitric oxide. Moreover, the findings indicate the enhancement of lipid peroxidation with the progression of the neoplastic process in B-CLL patients.
本研究旨在调查中性粒细胞产生一氧化氮（NO）、环磷酸鸟苷（cGMP）和超氧阴离子自由基的情况，并检测B细胞慢性淋巴细胞白血病（B-CLL）患者血清或血浆中总一氧化氮、丙二醛（MDA）和cGMP水平的变化。
从20例B-CLL患者中分离出多形核中性粒细胞（PMNs）。采用格里斯方法测定细胞上清液和血清中的总亚硝酸盐。利用细胞色素c还原试验评估细胞中超氧阴离子自由基的产生。采用酶联免疫吸附测定试剂盒评估细胞上清液和血浆中的cGMP水平，而血清MDA水平则采用Guege和Aus的分光光度法进行测定。
B-CLL患者的PMNs具有NO生成受损和cGMP产生增强的特征。与对照组相反，重组人白细胞介素-15（rhlL-15）和重组人白细胞介素-18（rhIL-18）对PMNs释放这些介质没有显著影响。PMNs释放的超氧阴离子自由基减少。与对照组相比，B-CLL患者的血清MDA和血浆cGMP水平升高。
B-CLL患者的PMNs产生一氧化氮和超氧阴离子自由基减少可能会损害中性粒细胞对白血病B细胞的细胞毒性作用。PMNs分泌一氧化氮水平低且PMN上清液中cGMP水平高表明，在这些患者中，除一氧化氮外的其他物质可能会激活鸟苷酸环化酶（sGC）。此外，研究结果表明，随着B-CLL患者肿瘤进程的进展，脂质过氧化作用增强。
