Lierz Michael, Hafez Hafez M, Klopfleisch Robert, Lüschow Dörte, Prusas Christine, Teifke Jens P, Rudolf Miriam, Grund Christian, Kalthoff Donata, Mettenleiter Thomas, Beer Martin, Hardert Timm
Institute for Poultry Disease, Freie Universität, Berlin, Germany.
Emerg Infect Dis. 2007 Nov;13(11):1667-74. doi: 10.3201/eid1311.070705.
Because fatal infections with highly pathogenic avian influenza A (HPAI) virus subtype H5N1 have been reported in birds of prey, we sought to determine detailed information about the birds' susceptibility and protection after vaccination. Ten falcons vaccinated with an inactivated influenza virus (H5N2) vaccine seroconverted. We then challenged 5 vaccinated and 5 nonvaccinated falcons with HPAI (H5N1). All vaccinated birds survived; all unvaccinated birds died within 5 days. For the nonvaccinated birds, histopathologic examination showed tissue degeneration and necrosis, immunohistochemical techniques showed influenza virus antigen in affected tissues, and these birds shed high levels of infectious virus from the oropharynx and cloaca. Vaccinated birds showed no influenza virus antigen in tissues and shed virus at lower titers from the oropharynx only. Vaccination could protect these valuable birds and, through reduced virus shedding, reduce risk for transmission to other avian species and humans.
由于已有关于高致病性甲型禽流感（HPAI）病毒H5N1亚型感染猛禽致死的报道，我们试图确定有关鸟类接种疫苗后的易感性和保护性的详细信息。10只接种了灭活流感病毒（H5N2）疫苗的猎鹰产生了血清转化。然后，我们用HPAI（H5N1）对5只接种疫苗和5只未接种疫苗的猎鹰进行了攻毒。所有接种疫苗的鸟类存活；所有未接种疫苗的鸟类在5天内死亡。对于未接种疫苗的鸟类，组织病理学检查显示组织变性和坏死，免疫组化技术显示受影响组织中有流感病毒抗原，并且这些鸟类从口咽和泄殖腔排出高滴度的传染性病毒。接种疫苗的鸟类组织中未显示流感病毒抗原，仅从口咽排出较低滴度的病毒。接种疫苗可以保护这些珍贵的鸟类，并通过减少病毒排出，降低传播给其他鸟类和人类的风险。
