Khan Shams Tabrez, Takaichi Shinichi, Harayama Shigeaki
Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8, Kazusakamatari, Kisarazu-shi, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):383-6. doi: 10.1099/ijs.0.65103-0.
Two novel marine, Gram-negative, non-motile, catalase- and oxidase-positive, aerobic bacteria were isolated from coastal seawater in Tokyo Bay. Analysis of almost-complete 16S rRNA gene sequences showed that the two isolates are members of the genus Paracoccus, sharing highest 16S rRNA gene sequence similarity (96.5 %) with Paracoccus aminophilus NBRC 16710(T). The DNA-DNA reassociation values between P. aminophilus NBRC 16710(T) and these isolates were only 10-20 %, in contrast to the high DNA relatedness between the two isolates (89 %). At least 1 % (w/v) NaCl was required for growth. Cellular fatty acid profiles revealed C(18 : 1)omega7c as the major component and C(10 : 0) 3-OH as the major hydroxy fatty acid. Ubiquinone-10 was detected as the major respiratory quinone. The G+C content of the genomic DNA of both strains was 69 mol%. On the basis of DNA-DNA hybridization data and physiological and chemotaxonomic characteristics, it is proposed that these strains should be placed in a novel species, Paracoccus marinus sp. nov. The type strain is KKL-A5(T) (=NBRC 100637(T) =CIP 108500(T)); KKL-B9 (=NBRC 100640) is a reference strain.
从东京湾的沿海海水中分离出两株新型海洋革兰氏阴性、无运动性、过氧化氢酶和氧化酶阳性的需氧细菌。对近乎完整的16S rRNA基因序列分析表明，这两株分离菌属于副球菌属，与嗜氨基副球菌NBRC 16710(T)的16S rRNA基因序列相似度最高（96.5%）。嗜氨基副球菌NBRC 16710(T)与这些分离菌之间的DNA-DNA重结合值仅为10%-20%，而两株分离菌之间的DNA相关性较高（89%）。生长需要至少1%（w/v）的NaCl。细胞脂肪酸谱显示C(18 : 1)ω7c为主要成分，C(10 : 0) 3-OH为主要羟基脂肪酸。检测到泛醌-10为主要呼吸醌。两株菌株基因组DNA的G+C含量均为69 mol%。基于DNA-DNA杂交数据以及生理和化学分类特征，建议将这些菌株归为一个新物种，即海洋副球菌（Paracoccus marinus sp. nov.）。模式菌株为KKL-A5(T)（=NBRC 100637(T) =CIP 108500(T)）；KKL-B9（=NBRC 100640）为参考菌株。
