Li Hai-Feng, Qu Jian-Hang, Yang Jin-Shui, Li Zhi-Jian, Yuan Hong-Li
China Agricultural University, Beijing 100193, People's Republic of China.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2670-4. doi: 10.1099/ijs.0.004705-0. Epub 2009 Jul 22.
A Gram-negative, short ovoid- to coccus-shaped, aerobic, motile, non-spore-forming bacterium (designated strain KS-11(T)) was isolated from sediment of the eutrophic Guanting reservoir in Beijing, China. Colonies grown on R2A agar plates were circular, convex and colourless to orange. The strain grew in the presence of up to 1 % NaCl (optimum, 0 % NaCl). Growth occurred at 25-40 degrees C (optimum, 28-37 degrees C) and at pH 6.0-9.5 (optimum, pH 7.5-9.0). On the basis of 16S rRNA gene sequence similarity, strain KS-11(T) was shown to belong to the class Alphaproteobacteria, being closely related to Paracoccus marinus (96.9 % 16S rRNA gene sequence similarity), followed by Paracoccus koreensis (96.8 %), Paracoccus solventivorans (96.8 %), Paracoccus alkenifer (96.2 %) and Paracoccus kocurii (95.8 %). The major fatty acids of strain KS-11(T) were summed feature 7 (C(18 : 1)omega7c/omega9t/omega12t) (83.8 %) and C(18 : 0) (6.5 %) and the G+C content of the genomic DNA was 69.0 mol%. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that strain KS-11(T) represents a novel species of the genus Paracoccus, named Paracoccus chinensis sp. nov. The type strain is KS-11(T) (=CGMCC 1.7655(T)=NBRC 104937(T)).
从中国北京富营养化的官厅水库沉积物中分离出一株革兰氏阴性、短卵形至球形、需氧、运动、不形成芽孢的细菌（命名为菌株KS-11(T)）。在R2A琼脂平板上生长的菌落呈圆形、凸起，无色至橙色。该菌株在高达1% NaCl（最适为0% NaCl）的条件下生长。在25 - 40℃（最适为28 - 37℃）和pH 6.0 - 9.5（最适为pH 7.5 - 9.0）条件下生长。基于16S rRNA基因序列相似性，菌株KS-11(T)被证明属于α-变形菌纲，与海生副球菌（16S rRNA基因序列相似性为96.9%）密切相关，其次是韩国副球菌（96.8%）、解烃副球菌（96.8%）、产烯副球菌（96.2%）和科氏副球菌（95.8%）。菌株KS-11(T)的主要脂肪酸为总和特征7（C(18 : 1)ω7c/ω9t/ω12t）（83.8%）和C(18 : 0)（6.5%），基因组DNA的G + C含量为69.0 mol%。基于生理和化学分类数据的比较分析，建议菌株KS-11(T)代表副球菌属的一个新物种，命名为中华副球菌（Paracoccus chinensis sp. nov.）。模式菌株为KS-11(T)（=CGMCC 1.7655(T)=NBRC 104937(T)）。
