Weon Hang-Yeon, Kim Byung-Yong, Yoo Seung-Hee, Kwon Soon-Wo, Stackebrandt Erko, Go Seung-Joo
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):470-3. doi: 10.1099/ijs.0.65295-0.
Two yellow-pigmented bacterial strains, JS6-6(T) and JS17-8(T), isolated from soil samples from Jeju, Republic of Korea, were studied to determine their taxonomic positions. The cells of the two bacteria were aerobic, Gram-negative, non-motile, straight rods. 16S rRNA gene sequence analysis indicated that both isolates should be placed in the genus Chryseobacterium of the family Flavobacteriaceae. Their 16S rRNA gene sequences showed similarities of 93.7-97.5 % to those of type strains of the genus Chryseobacterium. The values for DNA-DNA relatedness between both strains and type strains of closely related Chryseobacterium species were below 34 %. The fatty acids of the novel strains were similar to those of species of the genus Chryseobacterium. Both strains had MK-6 as the predominant respiratory quinone. The DNA G+C contents of strains JS6-6(T) and JS17-8(T) were 39.9 and 41.4 mol%, respectively. Phylogenetic evidence, together with the DNA-DNA relatedness values and phenotypic characteristics, indicated that strains JS6-6(T) and JS17-8(T) represent two novel species of the genus Chryseobacterium, for which the names Chryseobacterium soli sp. nov. and Chryseobacterium jejuense sp. nov., respectively, are proposed. The type strains of Chryseobacterium soli sp. nov. and Chryseobacterium jejuense sp. nov. are JS6-6(T) (=KACC 12502(T)=DSM 19298(T)) and JS17-8(T) (=KACC 12501(T)=DSM 19299(T)), respectively.
对从韩国济州岛土壤样本中分离出的两株黄色色素细菌菌株JS6-6(T)和JS17-8(T)进行了研究，以确定它们的分类地位。这两种细菌的细胞为需氧、革兰氏阴性、无运动性的直杆状。16S rRNA基因序列分析表明，这两株分离菌都应归入黄杆菌科的金黄杆菌属。它们的16S rRNA基因序列与金黄杆菌属模式菌株的序列相似性为93.7 - 97.5%。这两株菌株与密切相关的金黄杆菌属物种的模式菌株之间的DNA-DNA相关性值均低于34%。新菌株的脂肪酸与金黄杆菌属物种的脂肪酸相似。两株菌株均以MK-6作为主要呼吸醌。菌株JS6-6(T)和JS17-8(T)的DNA G+C含量分别为39.9和41.4 mol%。系统发育证据以及DNA-DNA相关性值和表型特征表明，菌株JS6-6(T)和JS17-8(T)代表金黄杆菌属的两个新物种，分别命名为土壤金黄杆菌（Chryseobacterium soli sp. nov.）和济州金黄杆菌（Chryseobacterium jejuense sp. nov.）。土壤金黄杆菌（Chryseobacterium soli sp. nov.）和济州金黄杆菌（Chryseobacterium jejuense sp. nov.）的模式菌株分别为JS6-6(T)（=KACC 12502(T)=DSM 19298(T)）和JS17-8(T)（=KACC 12501(T)=DSM 19299(T)）。
