Weon Hang-Yeon, Noh Hyung-Jun, Son Jung-A, Jang Han Byul, Kim Byung-Yong, Kwon Soon-Wo, Stackebrandt Erko
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Feb;58(Pt 2):474-8. doi: 10.1099/ijs.0.65358-0.
An orange-coloured bacterial strain, designated 5715S-11(T), was isolated from an air sample in Suwon, Republic of Korea. The strain was strictly aerobic, Gram-negative, non-spore-forming, non-flagellated and rod-shaped, frequently forming filaments. Growth of the strain was observed at 4-40 degrees C (optimum, 30 degrees C), pH 6.0-8.0 (optimum, pH 7.0) and 0-2 % (w/v) NaCl. Phylogenetically, strain 5715S-11(T) was shown to be a member of the family Spirosomaceae within the phylum Bacteroidetes. Its closest relatives were Spirosoma linguale LMG 10896(T) (87.9 % 16S rRNA gene sequence similarity) and Larkinella insperata LMG 22510(T) (86.2 % 16S rRNA gene sequence similarity). Predominant cellular fatty acids were summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), C(16 : 1)omega5c and iso-C(15 : 0). Major polar lipids were phosphatidylethanolamine and unknown aminolipids and polar lipids. On the basis of the evidence from this polyphasic study, strain 5715S-11(T) is considered to represent a novel species of a new genus, for which the name Rudanella lutea gen. nov., sp. nov. is proposed. The type strain of Rudanella lutea is 5715S-11(T) (=KACC 12603(T) =DSM 19387(T)).
从韩国水原市的一个空气样本中分离出一株橙色细菌菌株，命名为5715S-11(T)。该菌株为严格需氧菌，革兰氏阴性，不产芽孢，无鞭毛，杆状，常形成丝状。该菌株在4-40℃（最适温度30℃）、pH 6.0-8.0（最适pH 7.0）和0-2%（w/v）NaCl条件下生长。系统发育分析表明，菌株5715S-11(T)是拟杆菌门螺旋体科的成员。其最接近的亲缘种是舌螺旋体LMG 10896(T)（16S rRNA基因序列相似性为87.9%）和隐秘拉金氏菌LMG 22510(T)（16S rRNA基因序列相似性为86.2%）。主要的细胞脂肪酸为总和特征3（C(16 : 1)ω7c和/或异-C(15 : 0) 2-OH）、C(16 : 1)ω5c和异-C(15 : 0)。主要极性脂为磷脂酰乙醇胺和未知氨基脂及极性脂。基于这一多相研究的证据表明，菌株5715S-11(T)被认为代表一个新属的一个新物种，为此提出新属名为鲁丹氏菌属（Rudanella），新种名为黄色鲁丹氏菌（Rudanella lutea）。黄色鲁丹氏菌的模式菌株为5715S-11(T)（=KACC 12603(T) =DSM 19387(T)）。
