Lee Jae-Jin, Srinivasan Sathiyaraj, Lim Sangyong, Joe Minho, Im Seonghun, Bae So Il, Park Kyoung Ryun, Han Ji-Hee, Park Se-Hee, Joo Bo-Min, Park Sol-Ji, Kim Myung Kyum
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
Curr Microbiol. 2014 Sep;69(3):286-91. doi: 10.1007/s00284-014-0584-x. Epub 2014 Apr 20.
A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5A(T), was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5A(T) belong to the genus Spirosoma in the family Spirosomaceae, and the highest sequence similarities were 95.5 % with Spirosoma linguale DSM 74(T), 93.4 % with Spirosoma rigui WPCB118(T), 92.8 % with Spirosoma luteum SPM-10(T), 92.7 % with Spirosoma spitsbergense SPM-9(T), and 91.9 % with Spirosoma panaciterrae Gsoil 1519(T). Strain DG5A(T) revealed resistance to gamma and UV radiation. Chemotaxonomic data showed that the most abundant fatty acids were summed feature C(16:1) ω7c/C(16:1) ω6c (36.90 %), C(16:1) ω5c (29.55 %), and iso-C(15:0) (14.78 %), and the major polar lipid was phosphatidylethanolamine (PE). The DNA G+C content of strain DG5A(T) was 49.1 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain DG5A(T) presents a novel species of the genus Spirosoma, for which the name Spirosoma radiotolerans sp. nov., is proposed. The type strain is DG5A(T) (=KCTC 32455(T) = JCM19447(T)).
从韩国稻田土壤中分离出一株革兰氏阴性、短杆状、具有滑行运动性的细菌菌株，命名为DG5A(T)。利用新分离菌株的16S rRNA基因序列进行系统发育分析表明，菌株DG5A(T)属于螺菌科螺菌属，与舌螺菌DSM 74(T)的序列相似性最高，为95.5%，与里氏螺菌WPCB118(T)的相似性为93.4%，与黄色螺菌SPM-10(T)的相似性为92.8%，与斯匹次卑尔根螺菌SPM-9(T)的相似性为92.7%，与人参螺菌Gsoil 1519(T)的相似性为91.9%。菌株DG5A(T)对γ射线和紫外线具有抗性。化学分类数据显示，最丰富的脂肪酸为总和特征C(16:1)ω7c/C(16:1)ω6c(36.90%)、C(16:1)ω5c(29.55%)和异C(15:0)(14.78%)，主要极性脂为磷脂酰乙醇胺(PE)。菌株DG5A(T)的DNA G+C含量为49.1 mol%。综合表型、系统发育和化学分类数据支持菌株DG5A(T)代表螺菌属的一个新物种，为此提出新名称耐辐射螺菌（Spirosoma radiotolerans sp. nov.）。模式菌株为DG5A(T)(=KCTC 32455(T)=JCM19447(T))。
