Wang Wei, Yao Sheyue, Qiu Bin, Chen Guonan
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fujian, China.
J Sep Sci. 2008 Feb;31(3):574-8. doi: 10.1002/jssc.200700538.
A simple, reliable, and reproducible method for separation and determination of five enkephalin-related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8-98.2%.
本文描述了一种基于毛细管电泳安培检测（AD）分离和测定五种脑啡肽相关肽的简单、可靠且可重复的方法。向碳盘电极施加1.0 V的电位，该电极在本系统中用作工作电极。在15 kV的施加电压下，五种化合物在含有2.5%（v/v）甲醇的20 mmol/L磷酸盐缓冲溶液（PBS，pH 7.6）中于18分钟内分离。五种脑啡肽的检测限为6.31至54.3 nmol/L。该方法成功应用于测定添加到人脑脊液（CSF）中的五种化合物，回收率在95.8 - 98.2%范围内。
