采用安培检测的毛细管电泳法分离和测定脑啡肽相关肽。

Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection.

作者信息

Wang Wei, Yao Sheyue, Qiu Bin, Chen Guonan

机构信息

Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fujian, China.

出版信息

J Sep Sci. 2008 Feb;31(3):574-8. doi: 10.1002/jssc.200700538.

DOI:10.1002/jssc.200700538

PMID:18219656

Abstract

A simple, reliable, and reproducible method for separation and determination of five enkephalin-related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8-98.2%.

摘要

本文描述了一种基于毛细管电泳安培检测（AD）分离和测定五种脑啡肽相关肽的简单、可靠且可重复的方法。向碳盘电极施加1.0 V的电位，该电极在本系统中用作工作电极。在15 kV的施加电压下，五种化合物在含有2.5%（v/v）甲醇的20 mmol/L磷酸盐缓冲溶液（PBS，pH 7.6）中于18分钟内分离。五种脑啡肽的检测限为6.31至54.3 nmol/L。该方法成功应用于测定添加到人脑脊液（CSF）中的五种化合物，回收率在95.8 - 98.2%范围内。