Mahley R W, Weisgraber K H, Innerarity T
Biochemistry. 1976 Jul 13;15(14):2979-85. doi: 10.1021/bi00659a007.
Patas monkeys were studied for 2 years on three dietary regimes: (1) commercial chow (control diet); (2) semipurified diet plus lard (fat-fed); and (3) semipurified diet plus lard and cholesterol (cholesterol-fed). The control and fat-fed animals had similar lipoproteins which were equivalent to the human very low density, low density (LDL), and high density lipoproteins. An additional lipoprotein referred to as LDL-II appeared to be equivalent to the human Lp(a). The cholesterol-fed animals developed accelerated atherosclerosis associated with a hypercholesterolemia which was characterized by (1) the appearance of beta-migrating lipoproteins (B=VLDL) in the d less than 1.006, (2) an increase in the intermediate lipoproteins and LDL, and (3) the appearance of LDL-II which contained a prominence of the arginine-rich apoprotein. The arginine-rich apoprotein was also a prominent component of the B-VLDL and intermediate lipoproteins. Characterization of this apoprotein revealed that it contained 11.5 mol % arginine, had a molecular weight of approximately 34 000, and coelectrophoresed with the arginine-rich apoprotein of man, dog, swine, rat, and rabbit.
对猕猴进行了为期两年的研究，采用三种饮食方案：（1）商业饲料（对照饮食）；（2）半纯化饮食加猪油（高脂饮食）；（3）半纯化饮食加猪油和胆固醇（高胆固醇饮食）。对照动物和高脂饮食动物的脂蛋白相似，相当于人类的极低密度脂蛋白、低密度脂蛋白（LDL）和高密度脂蛋白。另一种称为LDL-II的脂蛋白似乎相当于人类的Lp(a)。高胆固醇饮食的动物出现了加速的动脉粥样硬化，伴有高胆固醇血症，其特征为：（1）在密度小于1.006的部分出现β迁移脂蛋白（B=VLDL）；（2）中间密度脂蛋白和LDL增加；（3）出现含有大量富含精氨酸载脂蛋白的LDL-II。富含精氨酸的载脂蛋白也是B-VLDL和中间密度脂蛋白的主要成分。对这种载脂蛋白的特性分析表明，它含有11.5摩尔%的精氨酸，分子量约为34000，与人、狗、猪、大鼠和兔子的富含精氨酸载脂蛋白共电泳。
