Rodemann H P, Peterson H P, Schwenke K, von Wangenheim K H
Developmental Biology Unit W7 and SFB 223, University of Bielefeld, FRG.
Scanning Microsc. 1991 Dec;5(4):1135-42; discussion 1142-3.
In order to analyze the effect of various kinds of radiation on the terminal differentiation processes of fibroblasts in culture, both human skin and lung fibroblasts were irradiated with electromagnetic non ionizing as well as ionizing radiation in clonal and sparse mass culture systems. As analyzed by cell biological (cell type frequencies), biochemical (collagen synthesis) and molecular markers (expression of protein PIVa) human skin and lung fibroblasts are induced to differentiate prematurely into terminal postmitotic cells. Thus, both electromagnetic and ionizing radiation induce terminal differentiation in cultured cells. These data add some new aspects for the interpretation of radiation effects on cells, e.g., in clinical therapy, as well as for the development of normal tissue responses during early and late effects after radiotherapy.
为了分析各种辐射对培养的成纤维细胞终末分化过程的影响，在克隆和稀疏群体培养系统中，用电磁非电离辐射以及电离辐射对人皮肤和肺成纤维细胞进行了照射。通过细胞生物学（细胞类型频率）、生物化学（胶原蛋白合成）和分子标志物（蛋白PIVa的表达）分析发现，人皮肤和肺成纤维细胞被诱导过早分化为终末有丝分裂后细胞。因此，电磁辐射和电离辐射均可诱导培养细胞的终末分化。这些数据为解释辐射对细胞的影响（如在临床治疗中）以及放疗后早期和晚期效应期间正常组织反应的发展增加了一些新的方面。
