Yuan Kun, Zhang Bo, Zhang Yanmei, Cheng Qiang, Wang Mingxiu, Huang Minren
Key Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University, Nanjing, China.
J Genet Genomics. 2008 Jan;35(1):49-60. doi: 10.1016/S1673-8527(08)60007-7.
Black spot disease in poplar is a disease of the leaf caused by fungus. The major pathogen is Marssonina brunnea f. sp. multigermtubi. To date, little is known about the molecular mechanism of poplar (M. brunnea) interaction. In order to identify the proteins related to disease resistance and understand its molecular basis, the clone "NL895" (P. euramericana CL"NL895"), which is highly resistant to M. brunnea f. sp. multigermtubi, was used in this study. We used two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) to identify the proteins in poplar leaves that were differentially expressed in response to black spot disease pathogen, M. brunnea f. sp. multigermtubi. Proteins extracted from poplar leaves at 0, 12, 24, 48, and 72 h after pathogen-inoculation were separated by 2-DE. About 500 reproducible protein spots were detected, of which 40 protein spots displayed differential expression in levels and were subjected to Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) followed by database searching. According to the function, the identified proteins were sorted into five categories, that is, protein synthesis, metabolism, defense response and unclassified proteins.
杨树黑斑病是一种由真菌引起的叶片病害。主要病原菌是多芽管盘二孢菌（Marssonina brunnea f. sp. multigermtubi）。迄今为止，关于杨树（与多芽管盘二孢菌）相互作用的分子机制知之甚少。为了鉴定与抗病性相关的蛋白质并了解其分子基础，本研究使用了对多芽管盘二孢菌高度抗病的无性系“NL895”（欧美杨无性系“NL895”）。我们使用二维凝胶电泳（2-DE）和质谱（MS）来鉴定杨树叶片中因黑斑病病原菌多芽管盘二孢菌而差异表达的蛋白质。对接种病原菌后0、12、24、48和72小时的杨树叶片提取的蛋白质进行二维凝胶电泳分离。检测到约500个可重复的蛋白质斑点，其中40个蛋白质斑点在水平上表现出差异表达，并进行了基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析，随后进行数据库搜索。根据功能，鉴定出的蛋白质分为五类，即蛋白质合成、代谢、防御反应和未分类蛋白质。
