Suppr超能文献

茉莉酸和乙烯诱导的线粒体交替氧化酶刺激杨树中 Marssonina brunnea 的防御反应。

Jasmonic Acid- and Ethylene-Induced Mitochondrial Alternative Oxidase Stimulates Marssonina brunnea Defense in Poplar.

机构信息

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, No. 159 Longpan Road, Nanjing, Jiangsu 210037, China.

出版信息

Plant Cell Physiol. 2021 Feb 4;61(12):2031-2042. doi: 10.1093/pcp/pcaa117.

Abstract

Mitochondrial processes are implicated in plant response to biotic stress caused by viruses, actinomyces, bacteria and pests, but their function in defense against fungal invasion remains unclear. Here, we investigated the role and regulation of mitochondrial alternative oxidase (AOX) in response to black spot disease caused by the hemibiotrophic fungus Marssonina brunnea in poplar. M. brunnea inoculation induced the transcription of the AOX1a gene in the mitochondrial electron transport chain and of jasmonic acid (JA) and ethylene (ET) biosynthetic genes, with the accumulation of these phytohormones in poplar leaf, while inhibiting the transcript amount of the mitochondrial cytochrome c oxidase gene (COX6b) and genes related to salicylic acid (SA). Enhanced AOX reduced poplar susceptibility to M. brunnea with a higher ATP/ADP ratio while the repressed AOX caused the reverse effect. Exogenous JA and 1-aminocyclopropane-1-carboxylic acid (ACC, a biosynthetic precursor of ET) inhibited the transcript amount of COX6b and consequently increased the ratio of AOX pathway to total respiration. Furthermore, the transcription of CYS C1 and CYS D1 genes catalyzing cyanide metabolism was induced, while the cysteine (CYS) substrate levels reduced upon M. brunnea inoculation; exogenous JA and ACC mimicked the effect of M. brunnea infection on cysteine. Exogenous SA enhanced, while JA and ACC reduced, poplar susceptibility to M. brunnea. Moreover, inhibiting AOX completely prohibited JA- and ET-increased tolerance to M. brunnea in poplar. These observations indicate that the JA- and ET-induced mitochondrial AOX pathway triggers defense against M. brunnea in poplar. This effect probably involves cyanide. These findings deepen our understanding of plant-pathogenic fungi interactions.

摘要

线粒体过程参与植物对病毒、放线菌、细菌和害虫等生物胁迫的反应,但它们在防御真菌入侵方面的功能仍不清楚。在这里,我们研究了线粒体交替氧化酶(AOX)在杨树对由半活体真菌 Marssonina brunnea 引起的黑斑病中的作用和调节。M. brunnea 接种诱导线粒体电子传递链中的 AOX1a 基因以及茉莉酸(JA)和乙烯(ET)生物合成基因的转录,杨树叶片中这些植物激素的积累,同时抑制线粒体细胞色素 c 氧化酶基因(COX6b)和与水杨酸(SA)相关的基因的转录量。增强的 AOX 降低了杨树对 M. brunnea 的易感性,同时提高了 ATP/ADP 比值,而抑制的 AOX 则产生相反的效果。外源 JA 和 1-氨基环丙烷-1-羧酸(ACC,ET 的生物合成前体)抑制 COX6b 的转录量,从而增加 AOX 途径与总呼吸的比值。此外,催化氰化物代谢的 CYS C1 和 CYS D1 基因的转录被诱导,而 M. brunnea 接种后半胱氨酸(CYS)底物水平降低;外源 JA 和 ACC 模拟了 M. brunnea 感染对半胱氨酸的影响。外源 SA 增强,而 JA 和 ACC 降低,杨树对 M. brunnea 的易感性。此外,完全抑制 AOX 可完全阻止 JA 和 ET 增加杨树对 M. brunnea 的耐受性。这些观察结果表明,JA 和 ET 诱导的线粒体 AOX 途径触发了杨树对 M. brunnea 的防御。这种效果可能涉及氰化物。这些发现加深了我们对植物-病原真菌相互作用的理解。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验