Harbers Matthias
DNAFORM, Inc., Leading Venture Plaza 2, 75-1 Ono-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0046, Japan.
Genomics. 2008 Mar;91(3):232-42. doi: 10.1016/j.ygeno.2007.11.004. Epub 2008 Jan 28.
The cloning of cDNAs, copies of cellular RNA, is one of the classical technologies in molecular biology. Over the past 30 years cDNA cloning technologies have been improved to enable the cloning of large cDNA collections, which are fundamental to today's understanding of the utilization of genetic information. With the discovery of noncoding RNAs, additional new approaches to the cloning of short RNAs have been developed. However, with the realization that much larger portions of genomes are transcribed than anticipated from genome annotations, cDNA cloning faces new challenges to uncover rare transcripts and to make the corresponding cDNAs available for functional studies. This review provides an overview on the current status of cDNA cloning and possibilities for the discovery and characterization of new RNA families.
细胞RNA的拷贝——cDNA的克隆是分子生物学中的经典技术之一。在过去30年里，cDNA克隆技术不断改进，能够克隆大型cDNA文库，这对于我们当今理解遗传信息的利用至关重要。随着非编码RNA的发现，又开发出了克隆短RNA的其他新方法。然而，随着人们认识到基因组中被转录的部分比基因组注释所预期的要大得多，cDNA克隆面临着新的挑战，即发现稀有转录本并获得相应的cDNA用于功能研究。本综述概述了cDNA克隆的现状以及发现新RNA家族并对其进行表征的可能性。
