CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Korea.
Exp Biol Med (Maywood). 2011 Sep;236(9):1070-7. doi: 10.1258/ebm.2011.011017. Epub 2011 Aug 1.
Mast cell-mediated allergic reaction is involved in many diseases such as asthma and allergic rhinitis. Therefore, discovery of drugs for the prevention or treatment of allergic disease is an important topic in human health. In this study, we evaluated the effects of water extract of Elsholtzia ciliata (Thunb.) Hyland (Labiatae) (WEEC) on mast cell-mediated allergic inflammation and studied the possible mechanisms of action. WEEC inhibited compound 48/80-induced systemic and immunoglobulin E-mediated local anaphylaxis, and serum histamine release in mice. WEEC reduced intracellular calcium levels and downstream histamine release from human mast cells (HMC-1) activated with phorbol 12-myristate 13-acetate and calcium ionophore A23187. In addition, WEEC decreased gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor-α, interleukin (IL)-1β and IL-6 in HMC-1. The inhibitory effect of WEEC on cytokine expression was nuclear factor (NF)-κB and p38 mitogen-activated protein kinase (MAPK) dependent. Our results indicate that WEEC inhibits mast cell-mediated allergic inflammatory reactions by suppressing histamine release and proinflammatory cytokine expression, and involvement of calcium, NF-κB and p38 MAPK in these effects.
肥大细胞介导的过敏反应涉及许多疾病，如哮喘和过敏性鼻炎。因此，发现预防或治疗过敏疾病的药物是人类健康的一个重要课题。在这项研究中，我们评估了香薷（Thunb.）水提取物（Labiatae）（WEEC）对肥大细胞介导的过敏炎症的影响，并研究了其可能的作用机制。WEEC 抑制了化合物 48/80 诱导的全身性和免疫球蛋白 E 介导的局部过敏反应，以及小鼠血清中的组胺释放。WEEC 降低了细胞内钙水平和佛波醇 12-肉豆蔻酸 13-乙酸和钙离子载体 A23187 激活的人肥大细胞（HMC-1）下游的组胺释放。此外，WEEC 减少了 HMC-1 中促炎细胞因子如肿瘤坏死因子-α、白细胞介素（IL）-1β和 IL-6 的基因表达和分泌。WEEC 对细胞因子表达的抑制作用依赖于核因子（NF）-κB 和 p38 丝裂原活化蛋白激酶（MAPK）。我们的结果表明，WEEC 通过抑制组胺释放和促炎细胞因子表达来抑制肥大细胞介导的过敏炎症反应，而钙、NF-κB 和 p38 MAPK 参与了这些作用。
