Sun Ying, Eichelbaum Ehrentraud J, Wang Hai, Vesely David L
Department of Internal Medicine, University of South Florida Cardiac Hormone Center and James A. Haley Veterans Medical Center, Tampa, FL 33612, USA.
Anticancer Res. 2007 Nov-Dec;27(6B):3813-8.
Atrial natriuretic peptide and long acting natriuretic peptide have anticancer effects in human prostate adenocarcinomas.
Atrial natriuretic peptide, long acting natriuretic peptide and cyclic GMP's effects on MEK 1/2 kinase were examined in human prostate adenocarcinoma cells.
Atrial natriuretic peptide and long acting natriuretic peptide decreased the activation of MEK 1/2 over a concentration range of 0.01 microM to 10 microM. Long acting natriuretic peptide and atrial natriuretic peptide (each 10 microM) inhibited the phosphorylation of MEK 1/2 kinase 97% (p < 0.00001) and 88% (p < 0.00001), respectively. The inhibition of MEK 1/2 was maximal at two hours, and ceased by four hours, secondary to both peptides. The ability of peptides to inhibit MEK 1/2 was inhibited by cyclic GMP antibody and cyclic GMP itself inhibited MEK 1/2 phosphorylation by 93%.
Atrial natriuretic peptide and long acting natriuretic peptide both inhibit MEK 1/2 kinase mediated via cyclic GMP as part of their anticancer mechanism(s) of action.
心房利钠肽和长效利钠肽在人类前列腺腺癌中具有抗癌作用。
在人类前列腺腺癌细胞中检测心房利钠肽、长效利钠肽和环磷酸鸟苷对MEK 1/2激酶的影响。
在0.01微摩尔至10微摩尔的浓度范围内，心房利钠肽和长效利钠肽降低了MEK 1/2的激活。长效利钠肽和心房利钠肽（各10微摩尔）分别抑制MEK 1/2激酶磷酸化97%（p < 0.00001）和88%（p < 0.00001）。两种肽对MEK 1/2的抑制在两小时时达到最大，四小时时停止。肽抑制MEK 1/2的能力被环磷酸鸟苷抗体抑制，且环磷酸鸟苷本身将MEK 1/2磷酸化抑制了93%。
心房利钠肽和长效利钠肽均通过环磷酸鸟苷介导抑制MEK 1/2激酶，这是它们抗癌作用机制的一部分。
