Gambichler Thilo, Schlaffke Axel, Tomi Nordwig S, Othlinghaus Nick, Altmeyer Peter, Kreuter Alexander
Department of Dermatology and Allergology, Ruhr-University Bochum, Gudrunstrasse 56, 44791 Bochum, Germany.
J Dermatol Sci. 2008 May;50(2):115-22. doi: 10.1016/j.jdermsci.2007.11.013. Epub 2008 Jan 15.
There is a lack of data with regard to the interaction between ultraviolet (UV) radiation and topical calcineurin inhibitors.
We aimed to investigate (1) the UV transmission through tacrolimus ointment and (2) the impact of topical exposure to tacrolimus on the protein expression of thymine dimers (TD) and p53 in human skin.
Spectrophotometric measurements (290-400 nm) of tacrolimus ointment and the vehicle were performed. Eight subjects were treated with tacrolimus ointment and the vehicle thrice daily over a 3-day period on the back. Pre-treated sites and one control site were exposed to two minimal erythema doses UVB. Skin biopsies were taken 1h and 24h after irradiation. Immunohistochemical procedures were used for the detection of TD and p53.
Mean UV transmission was over 94% and did not significantly differ between tacrolimus ointment and the vehicle. Immunohistological examinations of TD and p53 expression did not demonstrate significant differences between irradiated sites, irradiated plus vehicle treated sites, and irradiated plus tacrolimus treated sites both 1h and 24h post-irradiation.
The present data suggest that tacrolimus ointment hardly has UV blocking capacities and does not significantly interfere with development and/or removal of local DNA damage in human skin.
关于紫外线（UV）辐射与局部钙调神经磷酸酶抑制剂之间的相互作用，目前缺乏相关数据。
我们旨在研究（1）他克莫司软膏的紫外线透过率，以及（2）局部应用他克莫司对人皮肤中胸腺嘧啶二聚体（TD）和p53蛋白表达的影响。
对他克莫司软膏及其赋形剂进行分光光度测量（290 - 400nm）。8名受试者背部连续3天每天3次分别使用他克莫司软膏及其赋形剂。预处理部位和一个对照部位接受两次最小红斑量的UVB照射。照射后1小时和24小时取皮肤活检组织。采用免疫组织化学方法检测TD和p53。
平均紫外线透过率超过94%，他克莫司软膏与其赋形剂之间无显著差异。照射后1小时和24小时，TD和p53表达的免疫组织学检查显示，照射部位、照射加赋形剂处理部位以及照射加他克莫司处理部位之间无显著差异。
目前的数据表明，他克莫司软膏几乎没有紫外线阻挡能力，并且不会显著干扰人皮肤中局部DNA损伤的发生和/或清除。
