Unité de Recherche, Pathologies Humaines et Stress Oxydatif, Institut Supérieur de Biotechnologie de Sfax, Sfax, Tunisie.
Connect Tissue Res. 2010;51(1):55-8. doi: 10.3109/03008200902998709.
A critical step to escape from the carcinogenic potential of UV radiation is mediated by the protein p53. P53 activates growth arrest, allowing for DNA repair, which removes damaged cells. The concept of photoprotection involves blocking apoptosis and the prevention of oxidative damage to cellular DNA. Date seed oil (DSO) extract has been reported to be beneficial in the reduction of chemically induced oxidative stress in normal human skin. In this study, we investigated the DNA-protective qualities of DSO as measured by p53 expression in human skin biopsies, one day after exposure to ultraviolet B (UVB) radiation. P53 expression was analyzed using immunohistochemistry. The results showed an increase in p53 expression in the basal cell compartment of UVB-exposed skin as compared to the non-UVB-exposed skin. However, DSO has significant photoprotective effects by inhibition of damage caused by UVB irradiation: a significantly lower fraction of cells was p53 positive as compared to the non-DSO-treated skin. We conclude that p53 expression is a sensitive parameter for the detection of UVB-induced damage in the skin and suggest that DSO could provide an efficient complement to photoprotective measures and may contribute to reduce the DNA damage.
逃避紫外线致癌潜能的关键步骤是由蛋白质 p53 介导的。p53 激活生长停滞，允许 DNA 修复，从而去除受损细胞。光保护的概念涉及阻止细胞凋亡和防止细胞 DNA 的氧化损伤。据报道，芝麻籽油 (DSO) 提取物可减少正常人体皮肤中化学诱导的氧化应激。在这项研究中，我们通过分析人皮肤活检中 p53 表达，研究了 DSO 在紫外线 B (UVB) 辐射后一天的 DNA 保护特性。使用免疫组织化学分析 p53 表达。结果表明，与未暴露于 UVB 的皮肤相比，暴露于 UVB 的皮肤的基底层细胞中 p53 表达增加。然而，DSO 通过抑制 UVB 照射引起的损伤具有显著的光保护作用：与未用 DSO 处理的皮肤相比，p53 阳性细胞的比例显著降低。我们得出结论，p53 表达是检测皮肤中 UVB 诱导损伤的敏感参数，并表明 DSO 可以为光保护措施提供有效的补充，并可能有助于减少 DNA 损伤。
