Santus R, Perdrix L, Haigle J, Morliere P, Maziere J C, Maziere C, Labrid C
Muséum National d'Histoire Naturelle, Laboratoire de Physico-Chimie de l'Adaptation Biologique, Paris, France.
Photodermatol Photoimmunol Photomed. 1991 Oct;8(5):200-5.
Daflon is a strong inhibitor of Cu(2+)-induced arachidonic acid peroxidation, as revealed by the inhibition of thiobarbituric acid-reactive substance formation in mixed liposomes of phosphatidylcholine and arachidonic acid. Diosmin, the major Daflon constituent, is a good complexant of Cu2+ ions but not of Fe2+ ions. The Cu2+ complex formation may thus explain part of the antioxidant effect. However, Daflon is also a good quencher of the singlet oxygen-induced arachidonic acid peroxidation that does not involve metal ions. When cultured human fibroblasts are incubated with the diosmin-hesperidin mixture constituting Daflon, the latter is as efficient as vitamin E in inhibiting the ultraviolet A-induced lipid peroxidation and plasma membrane damage measured by lactate dehydrogenase release.
达夫隆是铜（2+）诱导的花生四烯酸过氧化的强效抑制剂，这在磷脂酰胆碱和花生四烯酸混合脂质体中硫代巴比妥酸反应性物质形成的抑制中得到了体现。地奥司明是达夫隆的主要成分，它是铜离子的良好络合剂，但不是亚铁离子的络合剂。因此，铜离子络合物的形成可能解释了部分抗氧化作用。然而，达夫隆也是单线态氧诱导的花生四烯酸过氧化的良好猝灭剂，这种过氧化不涉及金属离子。当培养的人成纤维细胞与构成达夫隆的地奥司明-橙皮苷混合物一起孵育时，后者在抑制紫外线A诱导的脂质过氧化和通过乳酸脱氢酶释放测量的质膜损伤方面与维生素E一样有效。
