Revermann Tobias, Götz Sebastian, Künnemeyer Jens, Karst Uwe
Chemical Analysis Group and MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands.
Analyst. 2008 Feb;133(2):167-74. doi: 10.1039/b711165g. Epub 2007 Nov 2.
Obstacles and possible solutions for the application of microchip capillary electrophoresis in quantitative analysis are described and critically discussed. Differences between the phenomena occurring during conventional capillary electrophoresis and microchip-based capillary electrophoresis are pointed out, with particular focus on electrolysis, bubble formation, clogging, surface interactions, injection and aspects related to the power supply. Current drawbacks are specified and improvements for successful quantitative microchip capillary electrophoresis are suggested.
描述并批判性地讨论了微芯片毛细管电泳在定量分析应用中的障碍及可能的解决方案。指出了传统毛细管电泳和基于微芯片的毛细管电泳过程中出现的现象之间的差异，特别关注电解、气泡形成、堵塞、表面相互作用、进样以及与电源相关的方面。明确了当前的缺点，并提出了实现成功的微芯片毛细管电泳定量分析的改进建议。
