Woods A, Couchman J R
University of Alabama at Birmingham, Birmingham, Alabama, USA.
Curr Protoc Cell Biol. 2001 May;Chapter 10:Unit 10.7. doi: 10.1002/0471143030.cb1007s07.
Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.
由于蛋白聚糖质量大、带电荷以及易于聚集或形成大分子复合物，它们可能是难以分离和分析的分子。本单元描述了纯化基质、细胞表面和细胞骨架连接的蛋白聚糖的详细方法。还描述了分析糖胺聚糖大小和类型以及核心蛋白种类的方法。
