Reinsch S
NASA-Ames Research Center, Moffett Field, California, USA.
Curr Protoc Cell Biol. 2001 May;Chapter 13:Unit 13.4. doi: 10.1002/0471143030.cb1304s10.
This unit describes the first assay that reconstructs the movement of the female pronucleus in the newly fertilized frog egg. Nuclei are assembled in frog egg extracts and translocated along microtubules using the microtubule motor dynein.
本单元描述了首个重建新受精蛙卵中雌性原核运动的实验方法。细胞核在蛙卵提取物中组装,并利用微管马达动力蛋白沿微管进行转运。