Detection and quantitation of radiolabeled proteins in gels and blots.

Permanent records of the results of electrophoretic separations of radiolabeled proteins and membrane-bound proteins (and RNA and DNA) can be made using autoradiography, fluorography, and phosphor imaging. These images can subsequently be quantified using densitometry to obtain a relative measure of the amount of radioactivity in a sample. This unit also includes descriptions of image-enhancement techniques and guidelines for selection of appropriate recording media.