Detection and quantitation of radiolabeled proteins in gels and blots.

This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifying screens and/or preflashing the film. Alternatively fluorography with organic scintillant-impregnated gels may be used to enhance signal. The autoradiogram may be quantified by densitometry. Phosphorimaging is a useful alternative to film exposure.